Biological Function of Pathogenesis-related Proteins: Four Tobacco Pathogenesis-related Proteins Are Chitinases

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1987 Oct 1

PMID 16578819

Citations 140

Authors

M Legrand

S Kauffmann

P Geoffroy

B Fritig

Affiliations

Soon will be listed here.

Abstract

Four endochitinases (poly[1,4-(N-acetyl-beta-D-glucosaminide)] glycanohydrolase, EC 3.2.1.14) have been purified from leaves of Nicotiana tabacum cv. Samsun NN reacting hypersensitively to tobacco mosaic virus. Two of them are acidic proteins of molecular weights 27,500 and 28,500 and have been identified as 2 of the 10 pathogenesis-related proteins that are known to accumulate in tobacco in response to stress or pathogen attack. These two pathogenesis-related proteins, named "P" and "Q" when their biological function was unknown, account for one-third of tobacco mosaic virus-induced chitinase activity of tobacco leaves. They are serologically closely related to the two other chitinases, which can be considered as new basic pathogenesis-related proteins. These two basic chitinases exhibit higher molecular weights (32,000 and 34,000) and higher specific enzyme activity than the two acidic isoforms.

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References

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Conejero V, Semancik J . Exocortis viroid: alteration in the proteins of Gynura aurantiaca accompanying viroid infection. Virology. 1977; 77(1):221-32. DOI: 10.1016/0042-6822(77)90420-2. View

Gianinazzi S, Martin C, Vallee J . [Hypersensitivity to viruses, temperature and soluble proteins in Nicotiana Xanthi n.c. Appearance of new macromolecules at the repression of viral synthesis]. C R Acad Hebd Seances Acad Sci D. 1970; 270(19):2383-6. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Morrissey J . Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem. 1981; 117(2):307-10. DOI: 10.1016/0003-2697(81)90783-1. View

Blake M, Johnston K, Russell-Jones G, Gotschlich E . A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal Biochem. 1984; 136(1):175-9. DOI: 10.1016/0003-2697(84)90320-8. View

Camacho Henriquez A, Sanger H . Analysis of acid-extractable tomato leaf proteins after infection with a viroid, two viruses and a fungus and partial purification of the "pathogenesis-related" protein p 14. Arch Virol. 1982; 74(2-3):181-96. DOI: 10.1007/BF01314711. View

REISSIG J, STORMINGER J, LELOIR L . A modified colorimetric method for the estimation of N-acetylamino sugars. J Biol Chem. 1955; 217(2):959-66. View

Hooft van Huijsduijnen R, Cornelissen B, van Loon L, VAN Boom J, Tromp M, Bol J . Virus-induced synthesis of messenger RNAs for precursors of pathogenesis-related proteins in tobacco. EMBO J. 1985; 4(9):2167-71. PMC: 554482. DOI: 10.1002/j.1460-2075.1985.tb03911.x. View

10.

Lucas J, Henriquez A, Lottspeich F, Henschen A, Sanger H . Amino acid sequence of the ;pathogenesis-related' leaf protein p14 from viroid-infected tomato reveals a new type of structurally unfamiliar proteins. EMBO J. 1985; 4(11):2745-9. PMC: 554573. DOI: 10.1002/j.1460-2075.1985.tb03998.x. View

11.

Cornelissen B, Hooft van Huijsduijnen R, van Loon L, Bol J . Molecular characterization of messenger RNAs for 'pathogenesis related' proteins la, lb and lc, induced by TMV infection of tobacco. EMBO J. 1986; 5(1):37-40. PMC: 1166692. DOI: 10.1002/j.1460-2075.1986.tb04174.x. View

12.

Mauch F, Hadwiger L, Boller T . Ethylene: Symptom, Not Signal for the Induction of Chitinase and beta-1,3-Glucanase in Pea Pods by Pathogens and Elicitors. Plant Physiol. 1984; 76(3):607-11. PMC: 1064341. DOI: 10.1104/pp.76.3.607. View

13.

Shinshi H, Mohnen D, Meins F . Regulation of a plant pathogenesis-related enzyme: Inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin. Proc Natl Acad Sci U S A. 1987; 84(1):89-93. PMC: 304147. DOI: 10.1073/pnas.84.1.89. View

14.

Somssich I, Schmelzer E, Bollmann J, Hahlbrock K . Rapid activation by fungal elicitor of genes encoding "pathogenesis-related" proteins in cultured parsley cells. Proc Natl Acad Sci U S A. 1986; 83(8):2427-30. PMC: 323310. DOI: 10.1073/pnas.83.8.2427. View

15.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

16.

Molano J, Polacheck I, Duran A, CABIB E . An endochitinase from wheat germ. Activity on nascent and preformed chitin. J Biol Chem. 1979; 254(11):4901-7. View

17.

Broglie K, Gaynor J, Broglie R . Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris. Proc Natl Acad Sci U S A. 1986; 83(18):6820-4. PMC: 386601. DOI: 10.1073/pnas.83.18.6820. View