Several "pathogenesis-related" Proteins in Potato Are 1,3-beta-glucanases and Chitinases

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1988 Feb 1

PMID 16578829

Citations 96

Authors

E Kombrink

M Schroder

K Hahlbrock

Affiliations

Soon will be listed here.

Abstract

Chitinase {poly[1,4-(N-acetyl-beta-D-glucosaminide)]glycanohydrolase, EC 3.2.1.14} and 1,3-beta-glucanase (1,3-beta-D-glucan 3-glucanohydrolase, EC 3.2.1.6) activities increased rapidly in potato (Solanum tuberosum) leaves inoculated with the pathogenic fungus Phytophthora infestans or treated with fungal elicitor. The enzyme activities were resolved into a total of two distinct 1,3-beta-glucanases and six proteins with chitinase activity. By several criteria, all of these proteins are classified as "pathogenesis-related" proteins whose biochemical functions have so far been unknown. Some of them constitute a major portion of the proteins accumulating in the intercellular space of infected potato leaves and are assumed to play an important role in pathogen defense.

Citing Articles

Lasiodiplodia theobromae protein LtScp1 contributes to fungal virulence and protects fungal mycelia against hydrolysis by grapevine chitinase.

Peng J, Li X, Li Y, Zhang W, Zhou Y, Yan J Environ Microbiol. 2022; 24(10):4670-4683.

PMID: 36054544 PMC: 9804331. DOI: 10.1111/1462-2920.16155.

Genes Associated with the Flax Plant Type (Oil or Fiber) Identified Based on Genome and Transcriptome Sequencing Data.

Povkhova L, Melnikova N, Rozhmina T, Novakovskiy R, Pushkova E, Dvorianinova E Plants (Basel). 2021; 10(12).

PMID: 34961087 PMC: 8707629. DOI: 10.3390/plants10122616.

Primes Onion Response against Stemphylium Leaf Blight Disease.

Roylawar P, Khandagale K, Randive P, Shinde B, Murumkar C, Ade A Pathogens. 2021; 10(9).

PMID: 34578118 PMC: 8472787. DOI: 10.3390/pathogens10091085.

Pathway-based analysis of anthocyanin diversity in diploid potato.

Parra-Galindo M, Soto-Sedano J, Mosquera-Vasquez T, Roda F PLoS One. 2021; 16(4):e0250861.

PMID: 33914830 PMC: 8084248. DOI: 10.1371/journal.pone.0250861.

Chitinase-like1 Plays a Role in Stalk Tensile Strength in Maize.

Jiao S, Hazebroek J, Chamberlin M, Perkins M, Sandhu A, Gupta R Plant Physiol. 2019; 181(3):1127-1147.

PMID: 31492738 PMC: 6836851. DOI: 10.1104/pp.19.00615.

References

Carr J, Dixon D, Klessig D . Synthesis of pathogenesis-related proteins in tobacco is regulated at the level of mRNA accumulation and occurs on membrane-bound polysomes. Proc Natl Acad Sci U S A. 1985; 82(23):7999-8003. PMC: 391429. DOI: 10.1073/pnas.82.23.7999. View

Shinshi H, Mohnen D, Meins F . Regulation of a plant pathogenesis-related enzyme: Inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin. Proc Natl Acad Sci U S A. 1987; 84(1):89-93. PMC: 304147. DOI: 10.1073/pnas.84.1.89. View

Boller T, Vogeli U . Vacuolar localization of ethylene-induced chitinase in bean leaves. Plant Physiol. 1984; 74(2):442-4. PMC: 1066699. DOI: 10.1104/pp.74.2.442. View

Lucas J, Henriquez A, Lottspeich F, Henschen A, Sanger H . Amino acid sequence of the ;pathogenesis-related' leaf protein p14 from viroid-infected tomato reveals a new type of structurally unfamiliar proteins. EMBO J. 1985; 4(11):2745-9. PMC: 554573. DOI: 10.1002/j.1460-2075.1985.tb03998.x. View

Cornelissen B, Hooft van Huijsduijnen R, van Loon L, Bol J . Molecular characterization of messenger RNAs for 'pathogenesis related' proteins la, lb and lc, induced by TMV infection of tobacco. EMBO J. 1986; 5(1):37-40. PMC: 1166692. DOI: 10.1002/j.1460-2075.1986.tb04174.x. View

Hooft van Huijsduijnen R, Cornelissen B, van Loon L, VAN Boom J, Tromp M, Bol J . Virus-induced synthesis of messenger RNAs for precursors of pathogenesis-related proteins in tobacco. EMBO J. 1985; 4(9):2167-71. PMC: 554482. DOI: 10.1002/j.1460-2075.1985.tb03911.x. View

SHUGAR D . The measurement of lysozyme activity and the ultra-violet inactivation of lysozyme. Biochim Biophys Acta. 1952; 8(3):302-9. DOI: 10.1016/0006-3002(52)90045-0. View

Carr J, Dixon D, Nikolau B, Voelkerding K, Klessig D . Synthesis and localization of pathogenesis-related proteins in tobacco. Mol Cell Biol. 1987; 7(4):1580-3. PMC: 365252. DOI: 10.1128/mcb.7.4.1580-1583.1987. View

Szewczyk B, KOZLOFF L . A method for the efficient blotting of strongly basic proteins from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose. Anal Biochem. 1985; 150(2):403-7. DOI: 10.1016/0003-2697(85)90528-7. View

10.

Camacho Henriquez A, Sanger H . Analysis of acid-extractable tomato leaf proteins after infection with a viroid, two viruses and a fungus and partial purification of the "pathogenesis-related" protein p 14. Arch Virol. 1982; 74(2-3):181-96. DOI: 10.1007/BF01314711. View

11.

HURN B, CHANTLER S . Production of reagent antibodies. Methods Enzymol. 1980; 70(A):104-42. DOI: 10.1016/s0076-6879(80)70044-7. View

12.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

13.

Molano J, Duran A, CABIB E . A rapid and sensitive assay for chitinase using tritiated chitin. Anal Biochem. 1977; 83(2):648-56. DOI: 10.1016/0003-2697(77)90069-0. View

14.

Lever M . A new reaction for colorimetric determination of carbohydrates. Anal Biochem. 1972; 47(1):273-9. DOI: 10.1016/0003-2697(72)90301-6. View

15.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

16.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

17.

Matsuoka M, Ohashi Y . Induction of pathogenesis-related proteins in tobacco leaves. Plant Physiol. 1986; 80(2):505-10. PMC: 1075144. DOI: 10.1104/pp.80.2.505. View

18.

Fritzemeier K, Cretin C, Kombrink E, Rohwer F, Taylor J, Scheel D . Transient Induction of Phenylalanine Ammonia-Lyase and 4-Coumarate: CoA Ligase mRNAs in Potato Leaves Infected with Virulent or Avirulent Races of Phytophthora infestans. Plant Physiol. 1987; 85(1):34-41. PMC: 1054198. DOI: 10.1104/pp.85.1.34. View

19.

Kombrink E, Hahlbrock K . Responses of cultured parsley cells to elicitors from phytopathogenic fungi : timing and dose dependency of elicitor-induced reactions. Plant Physiol. 1986; 81(1):216-21. PMC: 1075309. DOI: 10.1104/pp.81.1.216. View

20.

Somssich I, Schmelzer E, Bollmann J, Hahlbrock K . Rapid activation by fungal elicitor of genes encoding "pathogenesis-related" proteins in cultured parsley cells. Proc Natl Acad Sci U S A. 1986; 83(8):2427-30. PMC: 323310. DOI: 10.1073/pnas.83.8.2427. View