Isolation and Antigenic Characterization of Corn Mitochondrial F(1)-ATPase

Overview

Journal Plant Physiol

Specialty Physiology

Date 1985 Feb 1

PMID 16664055

Citations 6

Authors

V L Spitsberg

N E Pfeiffer

B Partridge

D E Wylie

S M Schuster

Affiliations

Soon will be listed here.

Abstract

Corn mitochondrial F(1)-ATPase was purified from submitochondrial particles by chloroform extraction. Enzyme stored in ammonium sulfate at 4 degrees C was substantially activated by ATP, while enzyme stored at -70 degrees C in 25% glycerol was not. Enzyme in glycerol remained fully active (8-9 micromoles P(i) released per minute per milligram), while the ammonium sulfate preparations steadily lost activity over a 2-month storage period. The enzyme was cold labile, and inactived by 4 minutes at 60 degrees C. Treatment with octylglucoside resulted in complete loss of activity, while vanadate had no effect on activity. The apparent subunit molecular weights of corn mitochondrial F(1)-ATPase were determined by SDS-polyacrylamide gel electrophoresis to be 58,000 (alpha), 55,000 (beta), 35,000 (gamma), 22,000 (delta), and 12,000 (epsilon). Monoclonal and polyclonal antibodies used in competitive binding assays demonstrated that corn mitochondrial F(1)-ATPase was antigenically distinct from the chloroplastic CF(1)-ATPases of corn and spinach. Monoclonal antibodies against antigenic sites on spinach CF(1)-ATPase beta and gamma subunits were used to demonstrate that those sites were either changed substantially or totally absent from the mitochondrial F(1)-ATPase.

Citing Articles

Intracellular sites of the synthesis of sweet potato mitochondrial F1ATPase subunits.

Iwasaki Y, Asahi T Plant Mol Biol. 2013; 5(6):339-46.

PMID: 24306987 DOI: 10.1007/BF00037554.

Purification and Characterization of the Soluble F(1)-ATPase of Oat Root Mitochondria.

Randall S, Wang Y, Sze H Plant Physiol. 1985; 79(4):957-62.

PMID: 16664552 PMC: 1075006. DOI: 10.1104/pp.79.4.957.

Nucleotide Sequence of the F(1)-ATPase alpha Subunit Gene from Maize Mitochondria.

Braun C, Levings C Plant Physiol. 1985; 79(2):571-7.

PMID: 16664454 PMC: 1074929. DOI: 10.1104/pp.79.2.571.

Kinetic Analysis of Corn Mitochondrial F(1)-ATPase.

Partridge B, Spitsberg V, Pfeiffer N, Schuster S Plant Physiol. 1985; 77(2):346-51.

PMID: 16664056 PMC: 1064517. DOI: 10.1104/pp.77.2.346.

Monoclonal Antibodies to the [alpha]- and [beta]-Subunits of the Plant Mitochondrial F1-ATPase.

Luethy M, Horak A, Elthon T Plant Physiol. 1993; 101(3):931-937.

PMID: 12231744 PMC: 158709. DOI: 10.1104/pp.101.3.931.

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