Intracellular Sites of the Synthesis of Sweet Potato Mitochondrial F1ATPase Subunits

Overview

Journal Plant Mol Biol

Date 2013 Dec 6

PMID 24306987

Authors

Y Iwasaki

T Asahi

Affiliations

Soon will be listed here.

Abstract

Five subunits (α-, β-, γ-, δ- and δ'-subunits) of the six α∼δ'-and ε-subunits) in the F1 portion (F1ATPase) of sweet potato (Ipomoea batatas) mitochondrial adenosine triphosphatase were isolated by an electrophoretic method. The δ- and δ'-subunits were not distinguishable immunologically but showed completely different tryptic peptide maps, indicating that they were different molecular species. In vitro protein synthesis with isolated sweet potato root mitochondria produced only the α-subunit when analyzed with anti-sweet potato F1ATPase antibody reacting with all the subunits except the ε-subunit. Sweet potato root poly(A)(+)RNA directed the synthesis of six polypeptides which were immunoprecipitated by the antibody: two of them immunologically related to the β-subunit and the others to the δ- and δ'-subunits. We conclude that the α-subunit of the F1ATPase is synthesized only in the mitochondria and the β-, δ- and δ'-subunits are in the cytoplasm.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Schatz G, Butow R . How are proteins imported into mitochondria?. Cell. 1983; 32(2):316-8. DOI: 10.1016/0092-8674(83)90450-6. View

Takeshima K, Nakano E . Modification of ribosomal proteins in sea urchin eggs following fertilization. Eur J Biochem. 1983; 137(3):437-43. DOI: 10.1111/j.1432-1033.1983.tb07847.x. View

Futai M, Kanazawa H . Structure and function of proton-translocating adenosine triphosphatase (F0F1): biochemical and molecular biological approaches. Microbiol Rev. 1983; 47(3):285-312. PMC: 281578. DOI: 10.1128/mr.47.3.285-312.1983. View

Watanabe A, Price C . Translation of mRNAs for subunits of chloroplast coupling factor 1 in spinach. Proc Natl Acad Sci U S A. 1982; 79(20):6304-8. PMC: 347109. DOI: 10.1073/pnas.79.20.6304. View

Leaver C, Hack E, Forde B . Protein synthesis by isolated plant mitochondria. Methods Enzymol. 1983; 97:476-84. DOI: 10.1016/0076-6879(83)97156-2. View

Spitsberg V, Pfeiffer N, Partridge B, Wylie D, Schuster S . Isolation and Antigenic Characterization of Corn Mitochondrial F(1)-ATPase. Plant Physiol. 1985; 77(2):339-45. PMC: 1064516. DOI: 10.1104/pp.77.2.339. View

Boutry M, Briquet M, Goffeau A . The alpha subunit of a plant mitochondrial F1-ATPase is translated in mitochondria. J Biol Chem. 1983; 258(14):8524-6. View

Iwasaki Y, Asahi T . Purification and characterization of the soluble form of mitochondrial adenosine triphosphatase from sweet potato. Arch Biochem Biophys. 1983; 227(1):164-73. DOI: 10.1016/0003-9861(83)90359-4. View

10.

Hattori T, Iwasaki Y, Sakajo S, Asahi T . Cell-free synthesis of succinate dehydrogenase and mitochondrial adenosine triphosphatase of sweet potato. Biochem Biophys Res Commun. 1983; 113(1):235-40. DOI: 10.1016/0006-291x(83)90456-4. View

11.

Hack E, Leaver C . The alpha-subunit of the maize F(1)-ATPase is synthesised in the mitochondrion. EMBO J. 1983; 2(10):1783-9. PMC: 555359. DOI: 10.1002/j.1460-2075.1983.tb01658.x. View

12.

Marcu K, Dudock B . Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ. Nucleic Acids Res. 1974; 1(11):1385-97. PMC: 343419. DOI: 10.1093/nar/1.11.1385. View

13.

Higgins R, Dahmus M . Rapid visualization of protein bands in preparative SDS-polyacrylamide gels. Anal Biochem. 1979; 93(2):257-60. DOI: 10.1016/s0003-2697(79)80148-7. View