Hydroxyproline-rich Bacterial Agglutinin from Potato : Extraction, Purification, and Characterization

Overview

Journal Plant Physiol

Specialty Physiology

Date 1982 Nov 1

PMID 16662679

Citations 39

Authors

J E Leach

M A Cantrell

L Sequeira

Affiliations

Soon will be listed here.

Abstract

A protein, extracted from Katahdin potato (Solanum tuberosum L. cv ;Katahdin') tubers and purified by ion exchange chromatography and gel filtration, agglutinates avirulent strains of the bacterial wilt pathogen, Pseudomonas solanacearum, but only weakly agglutinates virulent strains. The agglutinin has very low hemagglutinating activity (in contrast to potato lectin) and is a glycoprotein containing about 61% carbohydrate. The carbohydrate moiety contains 91% (weight%) arabinose, 5% galactose, 3% glucose, and 1% glucosamine. The protein portion is rich in hydroxyproline (42%), lysine (16%), serine (9%), and proline (9%). The entire agglutinin has a molecular weight of 91,000 +/- 5,000 and is very basic (pI > 11). Shape estimations based on the concentration dependence of the sedimentation coefficient, the high viscosity ([eta] = 92.7), the frictional coefficient (f/f(o) = 2.15), and axial ratio (a/b = 25) indicate that the agglutinin is a prolate ellipsoid.

Citing Articles

Plant Cell Walls Tackling Climate Change: Insights into Plant Cell Wall Remodeling, Its Regulation, and Biotechnological Strategies to Improve Crop Adaptations and Photosynthesis in Response to Global Warming.

Ezquer I, Salameh I, Colombo L, Kalaitzis P Plants (Basel). 2020; 9(2).

PMID: 32041306 PMC: 7076711. DOI: 10.3390/plants9020212.

Intracellular localization of posttranslational modifications in the synthesis of hydroxyproline-rich glycoproteins. Peptidyl proline hydroxylation in maize roots.

Sauer A, Robinson D Planta. 2013; 164(2):287-94.

PMID: 24249573 DOI: 10.1007/BF00396094.

Elicitor induction of the synthesis of a novel lectin-like arabinosylated hydroxyproline-rich glycoprotein in suspension cultures of Phaseolus vulgaris L.

Bolwell G Planta. 2013; 172(2):184-91.

PMID: 24225869 DOI: 10.1007/BF00394586.

Differential accumulation of hydroxyproline-rich glycoproteins in bean root nodule cells infected with a wild-type strain or a C4-dicarboxylic acid mutant of Rhizobium leguminosarum bv. phaseoli.

Benhamou N, Lafontaine P, Mazau D, Esquerre-Tugaye M Planta. 2013; 184(4):457-67.

PMID: 24194237 DOI: 10.1007/BF00197893.

Time-course study of the accumulation of hydroxyproline-rich glycoproteins in root cells of susceptible and resistant tomato plants infected by Fusarium oxysporum f. sp. radicis-lycopersici.

Benhamou N, Mazau D, Grenier J, Esquerre-Tugaye M Planta. 2013; 184(2):196-208.

PMID: 24194071 DOI: 10.1007/BF00197948.

References

Graham T, Sequeira L, Huang T . Bacterial lipopolysaccharides as inducers of disease resistance in tobacco. Appl Environ Microbiol. 1977; 34(4):424-32. PMC: 242674. DOI: 10.1128/aem.34.4.424-432.1977. View

Fairbanks G, Steck T, Wallach D . Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971; 10(13):2606-17. DOI: 10.1021/bi00789a030. View

Brysk M, Chrispeels M . Isolation and partial characterization of a hydroxyproline-rich cell wall glycoprotein and its cytoplasmic precursor. Biochim Biophys Acta. 1972; 257(2):421-32. DOI: 10.1016/0005-2795(72)90295-4. View

McCormick P, Chandrasekhar S, Millis A . Direct visualization of collagens and procollagens in polyacrylamide gels. Anal Biochem. 1979; 97(2):359-66. DOI: 10.1016/0003-2697(79)90086-1. View

Allen A, Neuberger A . The purification and properties of the lectin from potato tubers, a hydroxyproline-containing glycoprotein. Biochem J. 1973; 135(2):307-14. PMC: 1165824. DOI: 10.1042/bj1350307. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Esquerre-Tugaye M . Cell Surfaces in Plant-Microorganism Interactions: I. A Structural Investigation of Cell Wall Hydroxyproline-rich Glycoproteins Which Accumulate in Fungus-infected Plants. Plant Physiol. 1979; 64(2):314-9. PMC: 543078. DOI: 10.1104/pp.64.2.314. View

Stuart D, Varner J . Purification and Characterization of a Salt-extractable Hydroxyproline-rich Glycoprotein from Aerated Carrot Discs. Plant Physiol. 1980; 66(5):787-92. PMC: 440726. DOI: 10.1104/pp.66.5.787. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

10.

Gallop P . Studies on a parent gelatin from ichthyocol. Arch Biochem Biophys. 1955; 54(2):501-12. DOI: 10.1016/0003-9861(55)90062-x. View

11.

Marinkovich V . PURIFICATION AND CHARACTERIZATION OF THE HEMAGGLUTININ PRESENT IN POTATOES. J Immunol. 1964; 93:732-41. View

12.

Mort A, Lamport D . Anhydrous hydrogen fluoride deglycosylates glycoproteins. Anal Biochem. 1977; 82(2):289-309. DOI: 10.1016/0003-2697(77)90165-8. View

13.

Nishihara T, Doty P . THE SONIC FRAGMENTATION OF COLLAGEN MACROMOLECULES. Proc Natl Acad Sci U S A. 1958; 44(5):411-7. PMC: 335437. DOI: 10.1073/pnas.44.5.411. View

14.

EDELHOCH H . Spectroscopic determination of tryptophan and tyrosine in proteins. Biochemistry. 1967; 6(7):1948-54. DOI: 10.1021/bi00859a010. View

15.

Turner R, Liener I . The use of glutaraldehyde-treated erythrocytes for assaying the agglutinating activity of lectins. Anal Biochem. 1975; 68(2):651-3. DOI: 10.1016/0003-2697(75)90663-6. View

16.

Horejsi V, KOCOUREK J . Studies on lectins. XXXVII. Isolation and characterization of the lectin from Jimson-weed seeds (Datura stramonium L.). Biochim Biophys Acta. 1978; 532(1):92-7. View

17.

Allen A, Desai N, Neuberger A, CREETH J . Properties of potato lectin and the nature of its glycoprotein linkages. Biochem J. 1978; 171(3):665-74. PMC: 1184012. DOI: 10.1042/bj1710665. View