» Articles » PMID: 16665181

Detection of Glycosylated and Deglycosylated Extensin Precursors by Indirect Competitive ELISA

Overview
Journal Plant Physiol
Specialty Physiology
Date 1987 Jan 1
PMID 16665181
Citations 2
Authors
Affiliations
Soon will be listed here.
Abstract

A competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid quantitation of the glycosylated and deglycosylated forms of the monomeric soluble extensin precursor subunits P1 and P2. A log-linear response range for each kind of precursor in the competition curve was between 0.01 and 100 nanograms per milliliter.

Citing Articles

Antibody localization of extensin in cell walls of carrot storage roots.

Stafstrom J, Staehelin L Planta. 2013; 174(3):321-32.

PMID: 24221513 DOI: 10.1007/BF00959517.


Characterization of a xylose-specific antiserum that reacts with the complex asparagine-linked glycans of extracellular and vacuolar glycoproteins.

Lauriere M, Lauriere C, Chrispeels M, Johnson K, Sturm A Plant Physiol. 1989; 90(3):1182-8.

PMID: 16666870 PMC: 1061862. DOI: 10.1104/pp.90.3.1182.

References
1.
Stuart D, Varner J . Purification and Characterization of a Salt-extractable Hydroxyproline-rich Glycoprotein from Aerated Carrot Discs. Plant Physiol. 1980; 66(5):787-92. PMC: 440726. DOI: 10.1104/pp.66.5.787. View

2.
HEBERT G, Pelham P, Pittman B . Determination of the optimal ammonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera. Appl Microbiol. 1973; 25(1):26-36. PMC: 380730. DOI: 10.1128/am.25.1.26-36.1973. View

3.
Leach J, Cantrell M, Sequeira L . Hydroxyproline-rich bacterial agglutinin from potato : extraction, purification, and characterization. Plant Physiol. 1982; 70(5):1353-8. PMC: 1065887. DOI: 10.1104/pp.70.5.1353. View

4.
Lamport D, Miller D . Hydroxyproline arabinosides in the plant kingdom. Plant Physiol. 1971; 48(4):454-6. PMC: 396886. DOI: 10.1104/pp.48.4.454. View

5.
Vaitukaitis J, Robbins J, Nieschlag E, ROSS G . A method for producing specific antisera with small doses of immunogen. J Clin Endocrinol Metab. 1971; 33(6):988-91. DOI: 10.1210/jcem-33-6-988. View