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The Presence of Ribulose 1,5-diphosphate Carboxylase in the Nonphotosynthetic Endosperm of Germinating Castor Beans

Overview
Journal Plant Physiol
Specialty Physiology
Date 1973 Apr 1
PMID 16658404
Citations 12
Authors
C R Benedict
Affiliations
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Abstract

Ribulose 1,5-diphosphate carboxylase was detected in extracts of germinating castor bean (Ricinus communis var. Hale) endosperms. This is the first report of this enzyme in a nonphotosynthetic (no chlorophyll) plant tissue. Radioactive 3-phosphoglyceric acid has been identified as the principle product resulting from the enzymatic condensation of (14)C-bicarbonate and ribulose-1,5-diP in endosperm extracts. The Km values of bicarbonate and ribulose-1,5-diP for the endosperm carboxylase are 1.14 x 10(-2)m and 7.5 x 10(-5)m, respectively. The carboxylase activity peaks at 4 days in endosperms of castor beans germinated in the dark. The specific activity of the carboxylase at this stage of germination is 4.3 mumoles of 3-phosphoglycerate formed/mg protein.hr. The presence of ribulose-1,5-diP carboxylase and other enzymes of the reductive pentose phosphate pathway show the potential of this pathway in castor bean endosperms.

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