Uptake and Processing of the Precursor to the Small Subunit of Ribulose 1,5-bisphosphate Carboxylase by Leucoplasts from the Endosperm of Developing Castor Oil Seeds

Overview

Journal Plant Physiol

Specialty Physiology

Date 1986 Jul 1

PMID 16664909

Citations 18

Authors

S A Boyle

S M Hemmingsen

D T Dennis

Affiliations

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Abstract

Intact leucoplasts from the endosperm of developing castor oil seed were isolated by Percoll density gradient centrifugation. The precursor to the small subunit of ribulose 1,5-bisphosphate carboxylase from pea was synthesized in vitro from hybrid-selected mRNA. Leucoplasts imported this precursor by an ATP-requiring mechanism similar to that described in chloroplasts (AR Grossman et al. 1980 Nature 285: 625-628). The small subunit precursor was processed to a molecular weight that was identical with that of the mature pea small subunit. These results show that leucoplasts, though specialized for fatty acid biosynthesis and not photosynthesis, have a mechanism of protein import similar to that of chloroplasts.

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