Relationship of Histone Acetylation to DNA Topology and Transcription

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1991 Dec 1

PMID 1662766

Citations 11

Authors

W A Krajewski

A N Luchnik

Affiliations

Soon will be listed here.

Abstract

An autonomously replicating plasmid constructed from bovine papiloma virus (BPV) and pBR322 was stably maintained as a nuclear episome in a mouse cell culture. Addition to a cell culture of sodium butyrate (5 mM) induced an increase in plasmid DNA supercoiling of 3-5 turns, an increase in acetylation of cellular histones, and a decrease in plasmid transcription by 2- to 4-fold. After withdrawal of butyrate, DNA supercoiling began to fluctuate in a wave-like manner with an amplitude of up to 3 turns and a period of 3-4 h. These waves gradually faded by 24 h. The transcription of the plasmid and acetylation of cellular histones also oscillated with the same period. The wave-like alterations were not correlated with the cell cycle, for there was no resumption of DNA replication after butyrate withdrawal for at least 24 h. In vitro chemical acetylation of histones with acetyl adenylate also led to an increase in the superhelical density of plasmid DNA. The parallel changes in transcription, histone acetylation, and DNA supercoiling in vivo may indicate a functional innerconnection. Also, the observed in vivo variation in the level of DNA supercoiling directly indicates the possibility of its natural regulation in eukaryotic cells.

Citing Articles

Chromatin dynamics during DNA replication.

Bar-Ziv R, Voichek Y, Barkai N Genome Res. 2016; 26(9):1245-56.

PMID: 27225843 PMC: 5052047. DOI: 10.1101/gr.201244.115.

Effect of histone terminal domains on chromatin structural transitions.

Krajewski V, Lagarkova M, Sharova N, Stoliarov S, Ausio J Dokl Biochem Biophys. 2001; 377:132-4.

PMID: 11712144 DOI: 10.1023/a:1011556529349.

Ssn6-Tup1 interacts with class I histone deacetylases required for repression.

Watson A, Edmondson D, BONE J, Mukai Y, Yu Y, Du W Genes Dev. 2000; 14(21):2737-44.

PMID: 11069890 PMC: 317033. DOI: 10.1101/gad.829100.

Chromatin fiber structure: morphology, molecular determinants, structural transitions.

Zlatanova J, Leuba S, van Holde K Biophys J. 1998; 74(5):2554-66.

PMID: 9591681 PMC: 1299597. DOI: 10.1016/S0006-3495(98)77963-9.

Reconstitution of hyperacetylated, DNase I-sensitive chromatin characterized by high conformational flexibility of nucleosomal DNA.

Krajewski W, Becker P Proc Natl Acad Sci U S A. 1998; 95(4):1540-5.

PMID: 9465051 PMC: 19080. DOI: 10.1073/pnas.95.4.1540.

References

Ryoji M, WORCEL A . Structure of the two distinct types of minichromosomes that are assembled on DNA injected in Xenopus oocytes. Cell. 1985; 40(4):923-32. DOI: 10.1016/0092-8674(85)90352-6. View

Alfageme C, Zweidler A, Mahowald A, Cohen L . Histones of Drosophila embryos. Electrophoretic isolation and structural studies. J Biol Chem. 1974; 249(12):3729-36. View

Bode J, Schroter H . Nucleosomal particles open as the histone core becomes hyperacetylated. Eur J Biochem. 1983; 130(3):437-45. DOI: 10.1111/j.1432-1033.1983.tb07170.x. View

Allegra P, STERNER R, Clayton D, Allfrey V . Affinity chromatographic purification of nucleosomes containing transcriptionally active DNA sequences. J Mol Biol. 1987; 196(2):379-88. DOI: 10.1016/0022-2836(87)90698-x. View

Brill S, Sternglanz R . Transcription-dependent DNA supercoiling in yeast DNA topoisomerase mutants. Cell. 1988; 54(3):403-11. DOI: 10.1016/0092-8674(88)90203-6. View

Luchnik A, Hisamutdinov T, Georgiev G . Inhibition of transcription in eukaryotic cells by X-irradiation: relation to the loss of topological constraint in closed DNA loops. Nucleic Acids Res. 1988; 16(11):5175-90. PMC: 336726. DOI: 10.1093/nar/16.11.5175. View

Pruitt S, Reeder R . Effect of intercalating agents on RNA polymerase I promoter selection in Xenopus laevis. Mol Cell Biol. 1984; 4(12):2851-7. PMC: 369297. DOI: 10.1128/mcb.4.12.2851-2857.1984. View

Covault J, Sealy L, Schnell R, Shires A, Chalkley R . Histone hypoacetylation following release of HTC cells from butyrate. J Biol Chem. 1982; 257(10):5809-15. View

Spiker S . A modification of the acetic acid-urea system for use in microslab polyacrylamide gel electrophoresis. Anal Biochem. 1980; 108(2):263-5. DOI: 10.1016/0003-2697(80)90579-5. View

10.

Osborne B, Guarente L . Transcription by RNA polymerase II induces changes of DNA topology in yeast. Genes Dev. 1988; 2(6):766-72. DOI: 10.1101/gad.2.6.766. View

11.

Ramponi G, Manao G, Camici G . Nonenzymatic acetylation of histones with acetyl phosphate and acetyl adenylate. Biochemistry. 1975; 14(12):2681-5. DOI: 10.1021/bi00683a018. View

12.

Lescure B, Arcangioli B . Yeast RNA polymerase II initiates transcription in vitro at TATA sequences proximal to potential non-B forms of the DNA template. EMBO J. 1984; 3(5):1067-73. PMC: 557473. DOI: 10.1002/j.1460-2075.1984.tb01929.x. View

13.

Shen C, Hu W . DNA supercoiling of recombinant plasmids in mammalian cells. Proc Natl Acad Sci U S A. 1986; 83(6):1641-5. PMC: 323139. DOI: 10.1073/pnas.83.6.1641. View

14.

Pedone F, Filetici P, Ballario P . Yeast RNA polymerase II transcription of circular DNA at different degrees of supercoiling. Nucleic Acids Res. 1982; 10(17):5197-208. PMC: 320865. DOI: 10.1093/nar/10.17.5197. View

15.

Luchnik A, Bakayev V, Glaser V . DNA supercoiling: changes during cellular differentiation and activation of chromatin transcription. Cold Spring Harb Symp Quant Biol. 1983; 47 Pt 2:793-801. DOI: 10.1101/sqb.1983.047.01.091. View

16.

Luchnik A, Bakayev V, ZBARSKY I, Georgiev G . Elastic torsional strain in DNA within a fraction of SV40 minichromosomes: relation to transcriptionally active chromatin. EMBO J. 1982; 1(11):1353-8. PMC: 553216. DOI: 10.1002/j.1460-2075.1982.tb01322.x. View

17.

Weintraub H, Cheng P, Conrad K . Expression of transfected DNA depends on DNA topology. Cell. 1986; 46(1):115-22. DOI: 10.1016/0092-8674(86)90865-2. View

18.

POLACK A, Eick D, Koch E, Bornkamm G . Truncation does not abrogate transcriptional downregulation of the c-myc gene by sodium butyrate in Burkitt's lymphoma cells. EMBO J. 1987; 6(10):2959-64. PMC: 553732. DOI: 10.1002/j.1460-2075.1987.tb02601.x. View

19.

Krajewski W, Luchnik A . High rotational mobility of DNA in animal cells and its modulation by histone acetylation. Mol Gen Genet. 1991; 231(1):17-21. DOI: 10.1007/BF00293816. View

20.

Nelson D, Ferris R, Zhang D, Ferenz C . The beta-globin domain in immature chicken erythrocytes: enhanced solubility is coincident with histone hyperacetylation. Nucleic Acids Res. 1986; 14(4):1667-82. PMC: 339553. DOI: 10.1093/nar/14.4.1667. View