Effects of Histone Acetylation on Chromatin Topology in Vivo

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1992 Nov 1

PMID 1406675

Citations 14

Authors

L C Lutter

L Judis

R F Paretti

Affiliations

Soon will be listed here.

Abstract

Recently a model for eukaryotic transcriptional activation has been proposed in which histone hyperacetylation causes release of nucleosomal supercoils, and this unconstrained tension in turn stimulates transcription (V. G. Norton, B. S. Imai, P. Yau, and E. M. Bradbury, Cell 57:449-457, 1989; V. G. Norton, K. W. Marvin, P. Yau, and E. M. Bradbury, J. Biol. Chem. 265:19848-19852, 1990). These studies analyzed the effect of histone hyperacetylation on the change in topological linking number which occurs during nucleosome assembly in vitro. We have tested this model by determining the effect of histone hyperacetylation on the linking number change which occurs during assembly in vivo. We find that butyrate treatment of cells infected with simian virus 40 results in hyperacetylation of the histones of the extracted viral minichromosome as expected. However, the change in constrained supercoils of the minichromosome DNA is minimal, a result which is inconsistent with the proposed model. These results indicate that the proposed mechanism of transcriptional activation is unlikely to take place in the cell.

Citing Articles

Supercoiling in DNA and chromatin.

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Epigenetics of the depressed brain: role of histone acetylation and methylation.

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Role of histone N-terminal tails and their acetylation in nucleosome dynamics.

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Chromatin fiber structure: morphology, molecular determinants, structural transitions.

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References

Weintraub H . A dominant role for DNA secondary structure in forming hypersensitive structures in chromatin. Cell. 1983; 32(4):1191-203. DOI: 10.1016/0092-8674(83)90302-1. View

Panyim S, Chalkley R . High resolution acrylamide gel electrophoresis of histones. Arch Biochem Biophys. 1969; 130(1):337-46. DOI: 10.1016/0003-9861(69)90042-3. View

LABHART P, Koller T . Electron microscope specimen preparation of rat liver chromatin by a modified Miller spreading technique. Eur J Cell Biol. 1981; 24(2):309-16. View

Depew D, Wang J . Conformational fluctuations of DNA helix. Proc Natl Acad Sci U S A. 1975; 72(11):4275-9. PMC: 388703. DOI: 10.1073/pnas.72.11.4275. View

Keller W . Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis. Proc Natl Acad Sci U S A. 1975; 72(12):4876-80. PMC: 388835. DOI: 10.1073/pnas.72.12.4876. View

Schaffhausen B, BENJAMIN T . Deficiency in histone acetylation in nontransforming host range mutants of polyoma virus. Proc Natl Acad Sci U S A. 1976; 73(4):1092-6. PMC: 430206. DOI: 10.1073/pnas.73.4.1092. View

Riggs M, Whittaker R, Neumann J, Ingram V . n-Butyrate causes histone modification in HeLa and Friend erythroleukaemia cells. Nature. 1977; 268(5619):462-4. DOI: 10.1038/268462a0. View

Hagopian H, Riggs M, Swartz L, Ingram V . Effect of n-butyrate on DNA synthesis in chick fibroblasts and HeLa cells. Cell. 1977; 12(3):855-60. DOI: 10.1016/0092-8674(77)90284-7. View

Lutter L . Kinetic analysis of deoxyribonuclease I cleavages in the nucleosome core: evidence for a DNA superhelix. J Mol Biol. 1978; 124(2):391-420. DOI: 10.1016/0022-2836(78)90306-6. View

10.

Boffa L, Vidali G, Mann R, Allfrey V . Suppression of histone deacetylation in vivo and in vitro by sodium butyrate. J Biol Chem. 1978; 253(10):3364-6. View

11.

Candido E, Reeves R, Davie J . Sodium butyrate inhibits histone deacetylation in cultured cells. Cell. 1978; 14(1):105-13. DOI: 10.1016/0092-8674(78)90305-7. View

12.

Sealy L, Chalkley R . The effect of sodium butyrate on histone modification. Cell. 1978; 14(1):115-21. DOI: 10.1016/0092-8674(78)90306-9. View

13.

Mathis D, Oudet P, Wasylyk B, Chambon P . Effect of histone acetylation on structure and in vitro transcription of chromatin. Nucleic Acids Res. 1978; 5(10):3523-47. PMC: 342693. DOI: 10.1093/nar/5.10.3523. View

14.

Cousens L, Gallwitz D, Alberts B . Different accessibilities in chromatin to histone acetylase. J Biol Chem. 1979; 254(5):1716-23. View

15.

Finch J, Lutter L, Rhodes D, Brown R, Rushton B, Levitt M . Structure of nucleosome core particles of chromatin. Nature. 1977; 269(5623):29-36. DOI: 10.1038/269029a0. View

16.

Freeman L, Garrard W . DNA supercoiling in chromatin structure and gene expression. Crit Rev Eukaryot Gene Expr. 1992; 2(2):165-209. View

17.

Bonilla P, Freytag S, Lutter L . Enhancer-activated plasmid transcription complexes contain constrained supercoiling. Nucleic Acids Res. 1991; 19(14):3965-71. PMC: 328490. DOI: 10.1093/nar/19.14.3965. View

18.

Thomsen B, Bendixen C, Westergaard O . Histone hyperacetylation is accompanied by changes in DNA topology in vivo. Eur J Biochem. 1991; 201(1):107-11. DOI: 10.1111/j.1432-1033.1991.tb16262.x. View

19.

Krajewski W, Luchnik A . High rotational mobility of DNA in animal cells and its modulation by histone acetylation. Mol Gen Genet. 1991; 231(1):17-21. DOI: 10.1007/BF00293816. View

20.

Krajewski W, Luchnik A . Relationship of histone acetylation to DNA topology and transcription. Mol Gen Genet. 1991; 230(3):442-8. DOI: 10.1007/BF00280301. View