Requirement of Clathrin Heavy Chain for P53-mediated Transcription

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2006 Apr 19

PMID 16618797

Citations 26

Authors

Masato Enari

Kazuji Ohmori

Issay Kitabayashi

Yoichi Taya

Affiliations

Soon will be listed here.

Abstract

The p53 protein is a transcription factor that activates various genes responsible for growth arrest and/or apoptosis in response to DNA damage. Here, we report that clathrin heavy chain (CHC) binds to p53 and contributes to p53-mediated transcription. CHC is known to be a cytosolic protein that functions as a vesicle transporter. We found, however, that CHC exists not only in cytosol but also in nuclei. CHC expression enhances p53-dependent transactivation, whereas the reduction of CHC expression by RNA interference (RNAi) attenuates its transcriptional activity. Moreover, CHC binds to the p53-responsive promoter in vivo and stabilizes p53-p300 interaction to promote p53-mediated transcription. Thus, nuclear CHC is required for the transactivation of p53 target genes and plays a distinct role from clathrin-mediated endocytosis.

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