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Effects of Quercetin on Insulin-like Growth Factors (IGFs) and Their Binding Protein-3 (IGFBP-3) Secretion and Induction of Apoptosis in Human Prostate Cancer Cells

Overview
Journal J Carcinog
Publisher Biomed Central
Date 2006 Apr 8
PMID 16600019
Citations 14
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Abstract

Background: Quercetin, the predominant flavonoid, has been reported to lower the risk of several cancers. This flavonoid found in onion, grapes, green vegetables, etc. has been shown to possess potent antiproliferative effects against various malignant cells. This study was designed to investigate its effects on insulin-like growth factors (IGFs) and their binding protein-3 (IGFBP-3) proteins secretion and also apoptosis induction in the human prostate cancer cell line, PC-3.

Methods: We evaluated the secretion of IGF-I, -II and IGFBP-3 in quercetin treated cells by immunoradiometric (IRMA) method. Apoptosis was studied in quercetin treated cells by TUNEL and DNA fragmentation. Protein expressions of Bcl-2, Bcl-xL, Bax and caspase-3 were studied by western blot.

Results: At a dose of 100 microM concentration, we observed increased IGFBP-3 accumulation in PC-3 cells conditioned medium with a dose dependent increase with 2 fold over a base line, and significantly reduced the both IGF-I and IGF-II levels. Apoptosis induction was also confirmed by TUNEL assay. Bcl-2 and Bcl-xL protein expressions were significantly decreased and Bax and caspase-3 were increased.

Conclusion: These results suggest that the decreased level of IGFs could be due to the increased levels of IGFBP-3, because of the high binding affinity towards IGFs, thereby decreasing the cell proliferation. The increased level of IGFBP-3 was associated with increased pro-apoptotic proteins and apoptosis in response to quercetin, suggesting it may be a p53-independent effector of apoptosis in prostate cancer cells.

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References
1.
Rajah R, Khare A, Lee P, Cohen P . Insulin-like growth factor-binding protein-3 is partially responsible for high-serum-induced apoptosis in PC-3 prostate cancer cells. J Endocrinol. 1999; 163(3):487-94. DOI: 10.1677/joe.0.1630487. View

2.
Jackson J, White M, Yee D . Insulin receptor substrate-1 is the predominant signaling molecule activated by insulin-like growth factor-I, insulin, and interleukin-4 in estrogen receptor-positive human breast cancer cells. J Biol Chem. 1998; 273(16):9994-10003. DOI: 10.1074/jbc.273.16.9994. View

3.
Rajah R, Lee K, Cohen P . Insulin-like growth factor binding protein-3 mediates tumor necrosis factor-alpha-induced apoptosis: role of Bcl-2 phosphorylation. Cell Growth Differ. 2002; 13(4):163-71. View

4.
BUTT A, Firth S, BAXTER R . The IGF axis and programmed cell death. Immunol Cell Biol. 1999; 77(3):256-62. DOI: 10.1046/j.1440-1711.1999.00822.x. View

5.
Pollak M . Insulin-like growth factor physiology and cancer risk. Eur J Cancer. 2000; 36(10):1224-8. DOI: 10.1016/s0959-8049(00)00102-7. View