Accumulation of Hydroxyproline-rich Glycoprotein MRNAs in Response to Fungal Elicitor and Infection

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Oct 1

PMID 16593612

Citations 98

Authors

A M Showalter

J N Bell

C L Cramer

J A Bailey

J E Varner

C J Lamb

Affiliations

Soon will be listed here.

Abstract

Hydroxyproline-rich glycoproteins (HRGPs) are important structural components of plant cell walls and also accumulate in response to infection as an apparent defense mechanism. Accumulation of HRGP mRNA in biologically stressed bean (Phaseolus vulgaris L.) cells was monitored by blot hybridization with (32)P-labeled tomato genomic HRGP sequences. Elicitor treatment of suspension-cultured cells caused a marked increase in hybridizable HRGP mRNA. The response was less rapid but more prolonged than that observed for mRNAs encoding enzymes of phytoalexin biosynthesis. HRGP mRNA also accumulated during race:cultivar-specific interactions between bean hypocotyls and the partially biotrophic fungus Colletotrichum lindemuthianum, the causal agent of anthracnose. In an incompatible interaction (host resistant) there was an early increase in HRGP mRNA correlated with expression of hypersensitive resistance; whereas, in a compatible interaction (host susceptible), marked accumulation of HRGP mRNA occurred as a delayed response at the onset of lesion formation. In both interactions, mRNA accumulation was observed in uninfected cells distant from the site of fungal inoculation, indicating intercellular transmission of an elicitation signal.

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References

Chen J, Varner J . An extracellular matrix protein in plants: characterization of a genomic clone for carrot extensin. EMBO J. 1985; 4(9):2145-51. PMC: 554479. DOI: 10.1002/j.1460-2075.1985.tb03908.x. View

Roby D, Toppan A, Esquerre-Tugaye M . Cell surfaces in plant-microorganism interactions : v. Elicitors of fungal and of plant origin trigger the synthesis of ethylene and of cell wall hydroxyproline-rich glycoprotein in plants. Plant Physiol. 1985; 77(3):700-4. PMC: 1064587. DOI: 10.1104/pp.77.3.700. View

Chen J, Varner J . Isolation and characterization of cDNA clones for carrot extensin and a proline-rich 33-kDa protein. Proc Natl Acad Sci U S A. 1985; 82(13):4399-403. PMC: 390421. DOI: 10.1073/pnas.82.13.4399. View

Ryder T, Cramer C, Bell J, Robbins M, Dixon R, Lamb C . Elicitor rapidly induces chalcone synthase mRNA in Phaseolus vulgaris cells at the onset of the phytoalexin defense response. Proc Natl Acad Sci U S A. 1984; 81(18):5724-8. PMC: 391783. DOI: 10.1073/pnas.81.18.5724. View

Bell J, Dixon R, Bailey J, Rowell P, Lamb C . Differential induction of chalcone synthase mRNA activity at the onset of phytoalexin accumulation in compatible and incompatible plant-pathogen interactions. Proc Natl Acad Sci U S A. 1984; 81(11):3384-8. PMC: 345512. DOI: 10.1073/pnas.81.11.3384. View

Hahn M, Darvill A, Albersheim P . Host-Pathogen Interactions : XIX. THE ENDOGENOUS ELICITOR, A FRAGMENT OF A PLANT CELL WALL POLYSACCHARIDE THAT ELICITS PHYTOALEXIN ACCUMULATION IN SOYBEANS. Plant Physiol. 1981; 68(5):1161-9. PMC: 426062. DOI: 10.1104/pp.68.5.1161. View

Anderson-Prouty A, Albersheim P . Host-Pathogen Interactions: VIII. Isolation of a Pathogen-synthesized Fraction Rich in Glucan That Elicits a Defense Response in the Pathogen's Host. Plant Physiol. 1975; 56(2):286-91. PMC: 541806. DOI: 10.1104/pp.56.2.286. View

Dixon R, Dey P, Lawton M, Lamb C . Phytoalexin Induction in French Bean : Intercellular Transmission of Elicitation in Cell Suspension Cultures and Hypocotyl Sections of Phaseolus vulgaris. Plant Physiol. 1983; 71(2):251-6. PMC: 1066020. DOI: 10.1104/pp.71.2.251. View

Loschke D, Hadwiger L . Effects of Light and of Fusarium solani on Synthesis and Activity of Phenylalanine Ammonia-Lyase in Peas. Plant Physiol. 1981; 68(3):680-5. PMC: 425961. DOI: 10.1104/pp.68.3.680. View

10.

Ryan C, Bishop P, Pearce G . A sycamore cell wall polysaccharide and a chemically related tomato leaf polysaccharide possess similar proteinase inhibitor-inducing activities. Plant Physiol. 1981; 68(3):616-8. PMC: 425949. DOI: 10.1104/pp.68.3.616. View

11.

Leach J, Cantrell M, Sequeira L . Hydroxyproline-rich bacterial agglutinin from potato : extraction, purification, and characterization. Plant Physiol. 1982; 70(5):1353-8. PMC: 1065887. DOI: 10.1104/pp.70.5.1353. View

12.

Cramer C, Bell J, Ryder T, Bailey J, Schuch W, Bolwell G . Co-ordinated synthesis of phytoalexin biosynthetic enzymes in biologically-stressed cells of bean (Phaseolus vulgaris L.). EMBO J. 1985; 4(2):285-9. PMC: 554184. DOI: 10.1002/j.1460-2075.1985.tb03627.x. View

13.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

14.

. Rapid switching of plant gene expression induced by fungal elicitor. Science. 1985; 227(4691):1240-3. DOI: 10.1126/science.227.4691.1240. View

15.

Bolwell G, Robbins M, Dixon R . Metabolic changes in elicitor-treated bean cells. Enzymic responses associated with rapid changes in cell wall components. Eur J Biochem. 1985; 148(3):571-8. DOI: 10.1111/j.1432-1033.1985.tb08878.x. View

16.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

17.

Sarni F, Grand C, Boudet A . Purification and properties of cinnamoyl-CoA reductase and cinnamyl alcohol dehydrogenase from poplar stems (Populus X euramericana). Eur J Biochem. 1984; 139(2):259-65. DOI: 10.1111/j.1432-1033.1984.tb08002.x. View

18.

Sequeira L . Mechanisms of induced resistance in plants. Annu Rev Microbiol. 1983; 37:51-79. DOI: 10.1146/annurev.mi.37.100183.000411. View

19.

Dixon R, Dey P, Lamb C . Phytoalexins: enzymology and molecular biology. Adv Enzymol Relat Areas Mol Biol. 1983; 55:1-136. DOI: 10.1002/9780470123010.ch1. View

20.

Ebel J, Schmidt W, Loyal R . Phytoalexin synthesis in soybean cells: elicitor induction of phenylalanine ammonia-lyase and chalcone synthase mRNAs and correlation with phytoalexin accumulation. Arch Biochem Biophys. 1984; 232(1):240-8. DOI: 10.1016/0003-9861(84)90540-x. View