An Extracellular Matrix Protein in Plants: Characterization of a Genomic Clone for Carrot Extensin

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1985 Sep 1

PMID 15938047

Citations 107

Authors

J Chen

J E Varner

Affiliations

Soon will be listed here.

Abstract

Extensins are hydroxyproline-rich glycoproteins found in many plant cell walls as a major protein component. The peptide Ser-Hyp-Hyp-Hyp-Hyp is abundant in the extensins. Using extensin cDNA clones as probes, we isolated six different clones from carrot genomic libraries. One of the genomic clones, pDC5A1, was characterized and found to contain an open reading frame encoding extensin and a single intron in the 3'-non-coding region. The derived amino acid sequence contains a signal peptide sequence and 25 Ser-Pro-Pro-Pro-Pro repetitive sequences. Two extensin transcripts were found corresponding to pDC5A1 with different 5' start sites. These transcripts increased in abundance after wounding. This is consistent with the reported extensin accumulation in the cell wall upon wounding.

Citing Articles

Bioinformatic Identification and Analysis of Extensins in the Plant Kingdom.

Liu X, Wolfe R, Welch L, Domozych D, Popper Z, Showalter A PLoS One. 2016; 11(2):e0150177.

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Characterization of NtREL1, a novel root-specific gene from tobacco, and upstream promoter activity analysis in homologous and heterologous hosts.

Zhang C, Pan S, Chen H, Cai T, Zhuang C, Deng Y Plant Cell Rep. 2016; 35(4):757-69.

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Identification of the pI 4.6 extensin peroxidase from Lycopersicon esculentum using proteomics and reverse-genomics.

Dong W, Kieliszewski M, Held M Phytochemistry. 2014; 112:151-9.

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Plant gene expression in response to pathogens.

Collinge D, Slusarenko A Plant Mol Biol. 2013; 9(4):389-410.

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Molecular cloning of cDNAs encoding a putative cell wall protein from Zea mays and immunological identification of related polypeptides.

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