Suppressor Mutations in RpoA Suggest That OmpR Controls Transcription by Direct Interaction with the Alpha Subunit of RNA Polymerase

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 Dec 1

PMID 1657891

Citations 45

Authors

J M Slauch

F D Russo

T J Silhavy

Affiliations

Soon will be listed here.

Abstract

We have isolated mutations in rpoA, the gene encoding the alpha subunit of RNA polymerase, that specifically affect transcriptional control by OmpR and EnvZ, the two-component regulatory system that controls porin gene expression in Escherichia coli. Characterization of these mutations and a previously isolated rpoA allele suggests that both positive and negative regulation of porin gene transcription involves a direct interaction between OmpR and RNA polymerase through the alpha subunit. Several of the rpoA mutations cluster in the carboxy-terminal portion of the alpha protein, further suggesting that it is this domain of alpha that is involved in interaction with OmpR and perhaps other transcriptional regulators as well.

Citing Articles

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