Inhibitory Effect of Calcium-binding Protein Regucalcin on Ca2+/calmodulin-dependent Cyclic Nucleotide Phosphodiesterase Activity in Rat Liver Cytosol

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 1991 Jul 24

PMID 1656206

Citations 44

Authors

M Yamaguchi

H Tai

Affiliations

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Abstract

The effect of regucalcin, a calcium-binding protein isolated from rat liver cytosol, on Ca2+/calmodulin-dependent cyclic nucleotide (AMP) phosphodiesterase activity in rat liver cytosol was investigated. The addition of Ca2+ (50 microM) and calmodulin 160 U/ml in the enzyme reaction mixture caused a significant increase in cyclic AMP phosphodiesterase activity. This increase was inhibited by the presence of regucalcin (0.5-3.0 microM); the inhibitory effect was complete at 1.0 microM. Regucalcin (1.0 microM) did not have an appreciable effect on basal activity without Ca2+ and calmodulin. The inhibitory effect of regucalcin was still evident even at several fold higher concentrations of calmodulin (160-480 U/ml). However, regucalcin (1.0 microM) did not inhibit Ca2+/calmodulin-dependent cyclic AMP phosphodiesterase activity in the presence of 100 and 200 microM Ca2+ added. Meanwhile, Cd2+ (25-100 microM)-induced decrease in Ca2+/calmodulin-dependent cyclic AMP phosphodiesterase activity was not reversed by the presence of regucalcin (1.0 microM). The present results suggest that regucalcin can regulate Ca2+/calmodulin-dependent cyclic AMP phosphodiesterase activity due to binding Ca2+ in liver cells.

Citing Articles

The diverse roles of calcium-binding protein regucalcin in cell biology: from tissue expression and signalling to disease.

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Regucalcin and cell regulation: role as a suppressor protein in signal transduction.

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Stimulatory effect of regucalcin on proteolytic activity is impaired in the kidney cortex cytosol of rats with saline ingestion.

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Binding of kidney nuclear proteins to the 5'-flanking region of the rat gene for Ca2+-binding protein regucalcin: involvement of Ca2+/calmodulin signaling.

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Expression of calcium-binding protein regucalcin mRNA in the cloned rat hepatoma cells (H4-II-E) is stimulated through Ca2+ signaling factors: involvement of protein kinase C.

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