Potential Sensitivity of Hepatic Specific Protein Regucalcin As a Marker of Chronic Liver Injury

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 1997 Feb 1

PMID 9059996

Citations 9

Authors

M Yamaguchi

M Isogai

N Shimada

Affiliations

Soon will be listed here.

Abstract

A novel calcium-binding protein regucalcin has been shown to be specifically expressed in the liver of various specifies including human. Regucalcin concentration in the serum of patients with chronic liver injury was estimated by enzyme-linked immunoadsorbent assay (ELISA) with rabbit-anti-regucalcin IgG. Serum samples were obtained from 42 persons who were diagnosed as liver disorder. Serum regucalcin concentration in all patients was in the range of 3.7-69.6 ng/ml, although regucalcin was not entirely seen in the serum of normal subjects (10 persons) without hepatitis. Meanwhile, in 18 patients with liver injury, serum glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) activities were normal value (less than 40 I.U./I). Serum GOT and GPT activities from 24 patients showed a comparatively higher level (50-234 I.U./I). The present results demonstrate the potential sensitivity of regucalcin as a marker of chronic liver injury.

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References

Shimokawa N, Matsuda Y, Yamaguchi M . Genomic cloning and chromosomal assignment of rat regucalcin gene. Mol Cell Biochem. 1995; 151(2):157-63. DOI: 10.1007/BF01322338. View

Yamaguchi M, Oishi K, Isogai M . Expression of hepatic calcium-binding protein regucalcin mRNA is elevated by refeeding of fasted rats: involvement of glucose, insulin and calcium as stimulating factors. Mol Cell Biochem. 1995; 142(1):35-41. DOI: 10.1007/BF00928911. View

Yamaguchi M, Tai H . Inhibitory effect of calcium-binding protein regucalcin on Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase activity in rat liver cytosol. Mol Cell Biochem. 1991; 106(1):25-30. DOI: 10.1007/BF00231185. View

Mori S, Yamaguchi M . Hepatic calcium-binding protein regucalcin decreases Ca2+/calmodulin-dependent protein kinase activity in rat liver cytosol. Chem Pharm Bull (Tokyo). 1990; 38(8):2216-8. DOI: 10.1248/cpb.38.2216. View

Shimokawa N, Isogai M, Yamaguchi M . Specific species and tissue differences for the gene expression of calcium-binding protein regucalcin. Mol Cell Biochem. 1995; 143(1):67-71. DOI: 10.1007/BF00925928. View

Isogai M, Shimokawa N, Yamaguchi M . Hepatic calcium-binding protein regucalcin in released into the serum of rats administered orally carbon tetrachloride. Mol Cell Biochem. 1994; 131(2):173-9. DOI: 10.1007/BF00925954. View

Cheung W . Calmodulin plays a pivotal role in cellular regulation. Science. 1980; 207(4426):19-27. DOI: 10.1126/science.6243188. View

Isogai M, Oishi K, Yamaguchi M . Serum release of hepatic calcium-binding protein regucalcin by liver injury with galactosamine administration in rats. Mol Cell Biochem. 1994; 136(1):85-90. DOI: 10.1007/BF00931609. View

NISHIZUKA Y . Studies and perspectives of protein kinase C. Science. 1986; 233(4761):305-12. DOI: 10.1126/science.3014651. View

10.

Yamaguchi M, Kanayama Y, Shimokawa N . Expression of calcium-binding protein regucalcin mRNA in rat liver is stimulated by calcitonin: the hormonal effect is mediated through calcium. Mol Cell Biochem. 1994; 136(1):43-8. DOI: 10.1007/BF00931603. View

11.

Isogai M, Yamaguchi M . Calcium administration increases calcium-binding protein regucalcin concentration in the liver of rats. Mol Cell Biochem. 1995; 143(1):53-8. DOI: 10.1007/BF00925926. View

12.

Yamaguchi M, Mori S . Inhibitory effect of calcium-binding protein regucalcin on protein kinase C activity in rat liver cytosol. Biochem Med Metab Biol. 1990; 43(2):140-6. DOI: 10.1016/0885-4505(90)90019-w. View

13.

Yamaguchi M, Isogai M . Tissue concentration of calcium-binding protein regucalcin in rats by enzyme-linked immunoadsorbent assay. Mol Cell Biochem. 1993; 122(1):65-8. DOI: 10.1007/BF00925738. View

14.

Reinhart P, Taylor W, Bygrave F . The role of calcium ions in the mechanism of action of alpha-adrenergic agonists in rat liver. Biochem J. 1984; 223(1):1-13. PMC: 1144257. DOI: 10.1042/bj2230001. View

15.

Williamson J, Cooper R, Hoek J . Role of calcium in the hormonal regulation of liver metabolism. Biochim Biophys Acta. 1981; 639(3-4):243-95. DOI: 10.1016/0304-4173(81)90012-4. View

16.

Shimokawa N, Yamaguchi M . Calcium administration stimulates the expression of calcium-binding protein regucalcin mRNA in rat liver. FEBS Lett. 1992; 305(2):151-4. DOI: 10.1016/0014-5793(92)80884-j. View

17.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

18.

Yamaguchi M, Yamamoto T . Purification of calcium binding substance from soluble fraction of normal rat liver. Chem Pharm Bull (Tokyo). 1978; 26(6):1915-8. DOI: 10.1248/cpb.26.1915. View