Theileria Parva Candidate Vaccine Antigens Recognized by Immune Bovine Cytotoxic T Lymphocytes

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2006 Feb 24

PMID 16492763

Citations 59

Authors

Simon P Graham

Roger Pelle

Yoshikazu Honda

Duncan M Mwangi

Nyerhovwo J Tonukari

Mat Yamage

E Jane Glew

Etienne P de Villiers

Trushar Shah

Richard Bishop

Evelyne Abuya

Elias Awino

James Gachanja

Anthony E Luyai

Ferdinand Mbwika

Anthony M Muthiani

David M Ndegwa

Moses Njahira

John K Nyanjui

Fredrick O Onono

Julius Osaso

Rosemary M Saya

Claude Wildmann

Claire M Fraser

Ian Maudlin

Malcolm J Gardner

Subhash P Morzaria

Sheena Loosmore

Sarah C Gilbert

Jean-Christophe Audonnet

Pierre van der Bruggen

Vishvanath Nene

Evans L N Taracha

Affiliations

Soon will be listed here.

Abstract

East Coast fever, caused by the tick-borne intracellular apicomplexan parasite Theileria parva, is a highly fatal lymphoproliferative disease of cattle. The pathogenic schizont-induced lymphocyte transformation is a unique cancer-like condition that is reversible with parasite removal. Schizont-infected cell-directed CD8(+) cytotoxic T lymphocytes (CTL) constitute the dominant protective bovine immune response after a single exposure to infection. However, the schizont antigens targeted by T. parva-specific CTL are undefined. Here we show the identification of five candidate vaccine antigens that are the targets of MHC class I-restricted CD8(+) CTL from immune cattle. CD8(+) T cell responses to these antigens were boosted in T. parva-immune cattle resolving a challenge infection and, when used to immunize naïve cattle, induced CTL responses that significantly correlated with survival from a lethal parasite challenge. These data provide a basis for developing a CTL-targeted anti-East Coast fever subunit vaccine. In addition, orthologs of these antigens may be vaccine targets for other apicomplexan parasites.

Citing Articles

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