Vitrification of Mouse Embryos Using the Thin Plastic Strip Method

Overview

Journal Clin Exp Reprod Med

Date 2013 Jan 25

PMID 23346525

Citations 2

Authors

Eun Kyung Ryu

Yong Soo Hur

Ji Young Ann

Ja Young Maeng

Miji Park

Jeong Hyun Park

Jung Yoon

San Hyun Yoon

Chang Young Hur

Won Don Lee

Jin Ho Lim

Affiliations

Soon will be listed here.

Abstract

Objective: The aim of this study was to compare vitrification optimization of mouse embryos using electron microscopy (EM) grid, cryotop, and thin plastic strip (TPS) containers by evaluating developmental competence and apoptosis rates.

Methods: Mouse embryos were obtained from superovulated mice. Mouse cleavage-stage, expanded, hatching-stage, and hatched-stage embryos were cryopreserved in EM grid, cryotop, and TPS containers by vitrification in 15% ethylene glycol, 15% dimethylsulfoxide, 10 µg/mL Ficoll, and 0.65 M sucrose, and 20% serum substitute supplement (SSS) with basal medium, respectively. For the three groups in which the embryos were thawed in the EM grid, cryotop, and TPS containers, the thawing solution consisted of 0.25 M sucrose, 0.125 M sucrose, and 20% SSS with basal medium, respectively. Rates of survival, re-expansion, reaching the hatched stage, and apoptosis after thawing were compared among the three groups.

Results: Developmental competence after thawing of vitrified expanded and hatching-stage blastocysts using cryotop and TPS methods were significantly higher than survival using the EM grid (p<0.05). Also, apoptosis positive nuclei rates after thawing of vitrified expanded blastocysts using cryotop and TPS were significantly lower than when using the EM grid (p<0.05).

Conclusion: The TPS vitrification method has the advantages of achieving a high developmental ability and effective preservation.

Citing Articles

A retrospective study of single frozen-thawed blastocyst transfer.

Hur Y, Ryu E, Song S, Yoon S, Lim K, Lee W Clin Exp Reprod Med. 2016; 43(2):106-11.

PMID: 27358829 PMC: 4925865. DOI: 10.5653/cerm.2016.43.2.106.

Effect of micro-vibration culture system on embryo development.

Hur Y, Park J, Ryu E, Park S, Lee J, Lee S J Assist Reprod Genet. 2013; 30(6):835-41.

PMID: 23657828 PMC: 3696450. DOI: 10.1007/s10815-013-0007-0.

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