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A Mitochondrial Gene is Lost Via Homologous Recombination During Reversion of CMS T Maize to Fertility

Overview
Journal EMBO J
Date 1987 Jun 1
PMID 16453770
Citations 52
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Abstract

The Texas (T) male sterile cytoplasm of maize is distinguished by a mitochondrially synthesized 13-kd polypeptide and a high susceptibility to the toxin produced by the fungal pathogen Helminthosporium maydis. Fertile, toxin-resistant revertants show an altered restriction profile for mitochondrial DNA and do not produce the 13-kd polypeptide. Characterization of cosmid clones from CMS T maize and a revertant shows that a heavily transcribed open reading frame named T-URF13, potentially coding a 13-kd product, is deleted in the revertant mitochondria. Six transcripts present in CMS T mitochondria, 4000, 3000, 2000, 1800, 1500 and 1200 nucleotides in length, are lacking in revertant mitochondria. T-URF25, an open reading frame coding for a 25-kd product, lies to the 3' end of T-URF13 but is retained in the revertants. T-URF13 and T-URF25 are co-transcribed in CMS T mitochondria; in the revertant T-URF25 is present on a 3100-nucleotide species. The recombination that caused these changes involved a 127-bp repeated sequence. Homologous recombination took place within the central 55 bp of this imperfect repeat. Hybridization analysis of DNA and RNA from other revertants demonstrates that a similar or identical event has taken place independently in these revertants.

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References
1.
Levings C, Sederoff R . Nucleotide sequence of the S-2 mitochondrial DNA from the S cytoplasm of maize. Proc Natl Acad Sci U S A. 1983; 80(13):4055-9. PMC: 394199. DOI: 10.1073/pnas.80.13.4055. View

2.
Forde B, Oliver R, Leaver C . Variation in mitochondrial translation products associated with male-sterile cytoplasms in maize. Proc Natl Acad Sci U S A. 1978; 75(8):3841-5. PMC: 392883. DOI: 10.1073/pnas.75.8.3841. View

3.
SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

4.
Houchins J, Ginsburg H, Rohrbaugh M, Dale R, Schardl C, Hodge T . DNA sequence analysis of a 5.27-kb direct repeat occurring adjacent to the regions of S-episome homology in maize mitochondria. EMBO J. 1986; 5(11):2781-8. PMC: 1167223. DOI: 10.1002/j.1460-2075.1986.tb04568.x. View

5.
Messing J . New M13 vectors for cloning. Methods Enzymol. 1983; 101:20-78. DOI: 10.1016/0076-6879(83)01005-8. View