Article: Loss of CMS-specific mitochondrial DNA arrangement in fertile segregants of Petunia hybrids

The progeny of somatic hybrid Petunia plants derived from the fusion of a male-fertile line and a cytoplasmic male-sterile (cms) line were examined. Male-fertile progeny derived from three different male-sterile somatic hybrid plants did not exhibit the mitochondrial DNA (mtDNA) arrangement which has previously been correlated with cms in Petunia. The cms-associated mtDNA arrangement was present in the male-sterile predecessors of these fertile revertants. Thus, it is concluded that the loss of this mtDNA arrangement is associated with reversion to fertility in the progeny of the unstable somatic hybrid petunia plants.

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References

1.

Escote L, Gabay-Laughnan S, Laughnan J . Cytoplasmic reversion to fertility in cms-S maize need not involve loss of linear mitochondrial plasmids. Plasmid. 1985; 14(3):264-7. DOI: 10.1016/0147-619x(85)90011-3. View

2.

Dixon L, Leaver C, Brettell R, Gengenbach B . Mitochondrial sensitivity to Drechslera maydis T-toxin and the synthesis of a variant mitochondrial polypeptide in plants derived from maize tissue cultures with texas male-sterile cytoplasm. Theor Appl Genet. 2013; 63(1):75-80. DOI: 10.1007/BF00303496. View

3.

Brettell R, Thomas E, Ingram D . Reversion of Texas male-sterile cytoplasm maize in culture to give fertile, T-toxin resistant plants. Theor Appl Genet. 2013; 57(4):55-8. DOI: 10.1007/BF00277764. View

4.

Pring D, Levings C . Heterogeneity of Maize Cytoplasmic Genomes among Male-Sterile Cytoplasms. Genetics. 1978; 89(1):121-36. PMC: 1213822. DOI: 10.1093/genetics/89.1.121. View

5.

YOUNG E, Hanson M . A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated. Cell. 1987; 50(1):41-9. DOI: 10.1016/0092-8674(87)90660-x. View

6.

Levings 3rd C, Kim B, Pring D, Conde M, Mans R, Laughnan J . Cytoplasmic Reversion of cms-S in Maize: Association with a Transpositional Event. Science. 1980; 209(4460):1021-3. DOI: 10.1126/science.209.4460.1021. View

7.

Murray M, Thompson W . Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980; 8(19):4321-5. PMC: 324241. DOI: 10.1093/nar/8.19.4321. View

8.

Boeshore M, Hanson M, Izhar S . A variant mitochondrial DNA arrangement specific toPetunia stable sterile somatic hybrids. Plant Mol Biol. 2013; 4(2-3):125-32. DOI: 10.1007/BF02418759. View

9.

Dewey R, Levings 3rd C, Timothy D . Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell. 1986; 44(3):439-49. DOI: 10.1016/0092-8674(86)90465-4. View

10.

Rottmann W, Brears T, Hodge T, Lonsdale D . A mitochondrial gene is lost via homologous recombination during reversion of CMS T maize to fertility. EMBO J. 1987; 6(6):1541-6. PMC: 553522. DOI: 10.1002/j.1460-2075.1987.tb02398.x. View

11.

Kemble R, Mans R . Examination of the mitochondrial genome of revertant progeny from S cms maize with cloned S-1 and S-2 hybridization probes. J Mol Appl Genet. 1983; 2(2):161-71. View

Loss of CMS-specific Mitochondrial DNA Arrangement in Fertile Segregants of Petunia Hybrids