On the Mechanism of Cytoplasmic Male Sterility in the 447 Line of Vicia Faba

Overview

Journal Plant Mol Biol

Date 2013 Nov 27

PMID 24277621

Citations 10

Authors

T Turpen

S J Garger

L K Grill

Affiliations

Soon will be listed here.

Abstract

The trait of cytoplasmic male sterility, expressed in plants bearing the 447 cytoplasm of Vicia faba, is uniquely and positively correlated with the presence of a linear double-stranded RNA molecule (dsRNA) 16.7 kb in size. Restriction enzyme digestion profiles of mitochondrial DNA isolated from fertile and cytoplasmic malesterile (CMS) lines do show a limited number of specific differences in fragment intensities and mobilities. However, mitochondria isolated from the progeny of the cross CMS × Restorer line contain DNA with an identical restriction profile as the male-sterile parent: moreover, subsequent generations are completely and permanently fertile, even upon segregation of the nuclear restoration gene. Southern hybridizations, using cDNA clones as probes, reveal homology between the CMS-associated dsRNA and the nuclear genome of both sterile and fertile lines. The regions cloned, representing approximately 22% of the total dsRNA sequence, show no homology to organelle DNA. We have not been able to stably transmit the dsRNA to fertile lines of V. faba or any other plant species, using a variety of standard virological techniques.

Citing Articles

Successful full-length genomic cloning and characterization of site-specific nick structures of Phytophthora endornaviruses 2 and 3 in yeast, .

Sakuta K, Uchida K, Fukuhara T, Komatsu K, Okada R, Moriyama H Front Microbiol. 2023; 14:1243068.

PMID: 37771702 PMC: 10523305. DOI: 10.3389/fmicb.2023.1243068.

Two Novel Endornaviruses Co-infecting a Pathogen of Modulate the Developmental Stages and Fungicide Sensitivities of the Host Oomycete.

Uchida K, Sakuta K, Ito A, Takahashi Y, Katayama Y, Omatsu T Front Microbiol. 2021; 12:633502.

PMID: 33633714 PMC: 7902037. DOI: 10.3389/fmicb.2021.633502.

Endornaviruses: persistent dsRNA viruses with symbiotic properties in diverse eukaryotes.

Fukuhara T Virus Genes. 2019; 55(2):165-173.

PMID: 30644058 DOI: 10.1007/s11262-019-01635-5.

Double-stranded RNA and male sterility in rice.

Wang B, Li Y, Zhang X, Hu L, Wang J Theor Appl Genet. 2013; 79(4):556-60.

PMID: 24226462 DOI: 10.1007/BF00226167.

Differences between, and possible origins of, the cytoplasms found in fertile and male-sterile onions (Allium cepa L.).

Holford P, Croft J, Newbury H Theor Appl Genet. 2013; 82(6):737-44.

PMID: 24213449 DOI: 10.1007/BF00227319.

References

Ko K, Orfanides A, Straus N . A model for the evolution of the Vicia faba chloroplast genome. Theor Appl Genet. 2013; 74(1):125-39. DOI: 10.1007/BF00290095. View

Schardl C, Pring D, Lonsdale D . Mitochondrial DNA rearrangements associated with fertile revertants of S-type male-sterile maize. Cell. 1985; 43(1):361-8. DOI: 10.1016/0092-8674(85)90041-8. View

Boutry M, Briquet M . Mitochondrial modifications associated with the cytoplasmic male sterility in faba beans. Eur J Biochem. 1982; 127(1):129-35. DOI: 10.1111/j.1432-1033.1982.tb06846.x. View

Hull R, Covey S . Genome organization and expression of reverse transcribing elements: variations and a theme. J Gen Virol. 1986; 67 ( Pt 9):1751-8. DOI: 10.1099/0022-1317-67-9-1751. View

Duggleby R, Kinns H, Rood J . A computer program for determining the size of DNA restriction fragments. Anal Biochem. 1981; 110(1):49-55. DOI: 10.1016/0003-2697(81)90110-x. View

Wise R, Fliss A, Pring D, Gengenbach B . urf13-T of T cytoplasm maize mitochondria encodes a 13 kD polypeptide. Plant Mol Biol. 2013; 9(2):121-6. DOI: 10.1007/BF00015644. View

Finnegan P, Brown G . Autonomously replicating RNA in mitochondria of maize plants with S-type cytoplasm. Proc Natl Acad Sci U S A. 1986; 83(14):5175-9. PMC: 323913. DOI: 10.1073/pnas.83.14.5175. View

YOUNG E, Hanson M . A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated. Cell. 1987; 50(1):41-9. DOI: 10.1016/0092-8674(87)90660-x. View

Diaz-Ruiz J, Kaper J . Isolation of viral double-stranded RNAs using a LiCl fractionation procedure. Prep Biochem. 1978; 8(1):1-17. DOI: 10.1080/00327487808068215. View

10.

Weiner A, Deininger P, Efstratiadis A . Nonviral retroposons: genes, pseudogenes, and transposable elements generated by the reverse flow of genetic information. Annu Rev Biochem. 1986; 55:631-61. DOI: 10.1146/annurev.bi.55.070186.003215. View

11.

Grill L, Garger S . Identification and characterization of double-stranded RNA associated with cytoplasmic male sterility in Vicia faba. Proc Natl Acad Sci U S A. 1981; 78(11):7043-6. PMC: 349190. DOI: 10.1073/pnas.78.11.7043. View

12.

Dewey R, Levings 3rd C, Timothy D . Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell. 1986; 44(3):439-49. DOI: 10.1016/0092-8674(86)90465-4. View

13.

Harberd N, Flavell R, Thompson R . Identification of a transposon-like insertion in a Glu-1 allele of wheat. Mol Gen Genet. 1987; 209(2):326-32. DOI: 10.1007/BF00329661. View

14.

Turpen T, Garger S, Marks M, Grill L . Molecular cloning and physical characterization of a Brassica linear mitochondrial plasmid. Mol Gen Genet. 1987; 209(2):227-33. DOI: 10.1007/BF00329647. View

15.

Tsao S, Brunk C, Pearlman R . Hybridization of nucleic acids directly in agarose gels. Anal Biochem. 1983; 131(2):365-72. DOI: 10.1016/0003-2697(83)90185-9. View

16.

Schwarz-Sommer Z, Leclercq L, Gobel E, Saedler H . Cin4, an insert altering the structure of the A1 gene in Zea mays, exhibits properties of nonviral retrotransposons. EMBO J. 1987; 6(13):3873-80. PMC: 553864. DOI: 10.1002/j.1460-2075.1987.tb02727.x. View

17.

Wakarchuk D, Hamilton R . Cellular double-stranded RNA in Phaseolus vulgaris. Plant Mol Biol. 2013; 5(1):55-63. DOI: 10.1007/BF00017873. View

18.

Edwardson J, Bond D, Christie R . Cytoplasmic Sterility Factors in VICIA FABA L. Genetics. 1976; 82(3):443-9. PMC: 1213466. DOI: 10.1093/genetics/82.3.443. View

19.

Johns M, Mottinger J, Freeling M . A low copy number, copia-like transposon in maize. EMBO J. 1985; 4(5):1093-101. PMC: 554309. DOI: 10.1002/j.1460-2075.1985.tb03745.x. View

20.

Ie T . Cytoplasmic particles in Tropaeolum majus. Planta. 2014; 106(3):227-36. DOI: 10.1007/BF00388100. View