Amino Acid Sequence of the ;pathogenesis-related' Leaf Protein P14 from Viroid-infected Tomato Reveals a New Type of Structurally Unfamiliar Proteins

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1985 Nov 1

PMID 16453639

Citations 34

Authors

J Lucas

A C Henriquez

F Lottspeich

A Henschen

H L Sanger

Affiliations

Soon will be listed here.

Abstract

We have established the complete sequence of the 130 amino acid residues of the pathogenesis-related (PR) protein p14 accumulating in tomato leaves infected with the viroid of the spindle tuber disease of potato (PSTV) and partial sequences of the PR protein 1a which accumulates in tobacco mosaic virus-infected tobacco leaves. Both PR proteins are closely related to each other. However, no homology could be found between the sequence of p14 and any of the 3061 published protein sequences compiled in the protein sequence database at present. Thus, p14 represents not only the first completely sequenced PR plant protein but also a new type of structurally unfamiliar proteins whose biological function in the diseased plant remains to be elucidated.

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References

Chou P, Fasman G . Prediction of beta-turns. Biophys J. 1979; 26(3):367-83. PMC: 1328558. DOI: 10.1016/S0006-3495(79)85259-5. View

Chou P, Fasman G . Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol Relat Areas Mol Biol. 1978; 47:45-148. DOI: 10.1002/9780470122921.ch2. View

van Loon L . Induction by 2-chloroethylophosphonic acid of viral-like lesions, associated proteins, and systemic resistance in tobacco. Virology. 1977; 80(2):417-20. DOI: 10.1016/s0042-6822(77)80016-0. View

van Loon L, van Kammen A . Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. "Samsun" and "Samsun NN". II. Changes in protein constitution after infection with tobacco mosaic virus. Virology. 1970; 40(2):190-211. DOI: 10.1016/0042-6822(70)90395-8. View

EDMAN P, Begg G . A protein sequenator. Eur J Biochem. 1967; 1(1):80-91. DOI: 10.1007/978-3-662-25813-2_14. View

Abraham G, Podell D . Pyroglutamic acid. Non-metabolic formation, function in proteins and peptides, and characteristics of the enzymes effecting its removal. Mol Cell Biochem. 1981; 38 Spec No(Pt 1):181-90. DOI: 10.1007/BF00235695. View

Nishikawa K . Assessment of secondary-structure prediction of proteins. Comparison of computerized Chou-Fasman method with others. Biochim Biophys Acta. 1983; 748(2):285-99. DOI: 10.1016/0167-4838(83)90306-0. View

Nover L, Scharf K . Synthesis, modification and structural binding of heat-shock proteins in tomato cell cultures. Eur J Biochem. 1984; 139(2):303-13. DOI: 10.1111/j.1432-1033.1984.tb08008.x. View

Camacho Henriquez A, Sanger H . Analysis of acid-extractable tomato leaf proteins after infection with a viroid, two viruses and a fungus and partial purification of the "pathogenesis-related" protein p 14. Arch Virol. 1982; 74(2-3):181-96. DOI: 10.1007/BF01314711. View

10.

KYTE J, Doolittle R . A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982; 157(1):105-32. DOI: 10.1016/0022-2836(82)90515-0. View

11.

Nishikawa K, Ooi T . Prediction of the surface-interior diagram of globular proteins by an empirical method. Int J Pept Protein Res. 1980; 16(1):19-32. DOI: 10.1111/j.1399-3011.1980.tb02931.x. View

12.

Podell D, Abraham G . A technique for the removal of pyroglutamic acid from the amino terminus of proteins using calf liver pyroglutamate amino peptidase. Biochem Biophys Res Commun. 1978; 81(1):176-85. DOI: 10.1016/0006-291x(78)91646-7. View

13.

Nagano K . Triplet information in helix prediction applied to the analysis of super-secondary structures. J Mol Biol. 1977; 109(2):251-74. DOI: 10.1016/s0022-2836(77)80033-8. View

14.

Robson B, Suzuki E . Conformational properties of amino acid residues in globular proteins. J Mol Biol. 1976; 107(3):327-56. DOI: 10.1016/s0022-2836(76)80008-3. View

15.

Gianinazzi S, Martin C, Vallee J . [Hypersensitivity to viruses, temperature and soluble proteins in Nicotiana Xanthi n.c. Appearance of new macromolecules at the repression of viral synthesis]. C R Acad Hebd Seances Acad Sci D. 1970; 270(19):2383-6. View

16.

Camacho Henriquez A, Sanger H . Purification and partial characterization of the major "pathogenesis-related" tomato leaf protein P14 from potato spindle tuber viroid (PSTV)-infected tomato leaves. Arch Virol. 1984; 81(3-4):263-84. DOI: 10.1007/BF01309998. View

17.

Rawlings N, Ashman K, Wittmann-Liebold B . Computerised version of the Chou and Fasman protein secondary structure predictive method. Int J Pept Protein Res. 1983; 22(5):515-24. DOI: 10.1111/j.1399-3011.1983.tb02124.x. View

18.

Chang J . N-terminal sequence analysis of polypeptide at the picomole level. Biochem J. 1981; 199(3):557-64. PMC: 1163410. DOI: 10.1042/bj1990557. View

19.

Scharf K, Nover L . Heat-shock-induced alterations of ribosomal protein phosphorylation in plant cell cultures. Cell. 1982; 30(2):427-37. DOI: 10.1016/0092-8674(82)90240-9. View