Coding and 3' Non-coding Nucleotide Sequence of Chalcone Synthase MRNA and Assignment of Amino Acid Sequence of the Enzyme

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1983 Jan 1

PMID 16453477

Citations 70

Authors

U Reimold

M Kroger

F Kreuzaler

K Hahlbrock

Affiliations

Soon will be listed here.

Abstract

The nucleotide sequence of an almost complete cDNA copy of chalcone synthase mRNA from cultured parsley cells (Petroselinum hortense) has been determined. The cDNA copy comprised the complete coding sequence for chalcone synthase, a short A-rich stretch of the 5' non-coding region and the complete 3' non-coding region including a poly(A) tail. The amino acid sequence deduced from the nucleotide sequence of the cDNA is consistent with a partial N-terminal sequence analysis, the total amino acid composition, the cyanogen bromide cleavage pattern, and the apparent mol. wt. of the subunit of the purified enzyme.

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