Shotgun Lipidomics of Cardiolipin Molecular Species in Lipid Extracts of Biological Samples

Overview

Journal J Lipid Res

Publisher Elsevier

Specialty Biochemistry

Date 2006 Feb 2

PMID 16449763

Citations 86

Authors

Xianlin Han

Kui Yang

Jingyue Yang

Hua Cheng

Richard W Gross

Affiliations

Soon will be listed here.

Abstract

Cardiolipin is a prominent component of the mitochondrial inner membranes contributing to the regulation of multiple discrete mitochondrial functions. Here, we extend shotgun lipidomics to identify and quantitate cardiolipin molecular species directly from lipid extracts of biological samples. Three shotgun lipidomics approaches for analyses of cardiolipin molecular species were developed using either a continuous ion-transmission instrument (i.e., triple-quadrupole type) with either low or high mass resolution settings or a high mass resolution hybrid pulsed instrument [i.e., quadrupole time-of-flight (QqTOF) type]. Three chemical principles were used for the development of these approaches. These include the marked enrichment of linoleate in cardiolipin to maximize the signal-to-noise ratio, the specific neutral loss of ketenes from doubly charged cardiolipin molecular ions to yield doubly charged triacyl monolysocardiolipins, and the doubly charged character of two phosphates in each cardiolipin molecular species. Through these techniques, we identified and quantified the specific molecular species profiles of cardiolipin directly from lipid extracts of mouse heart, liver, and skeletal muscle. The accuracy ( approximately 5%) and the low end of the linear dynamic range (10 fmol/microl) for quantitation make these approaches useful for studying alterations in cardiolipin metabolism in multiple disease states using either type of mass spectrometer.

Citing Articles

Mitochondrial membrane lipids in the regulation of bioenergetic flux.

Decker S, Funai K Cell Metab. 2024; 36(9):1963-1978.

PMID: 39178855 PMC: 11374467. DOI: 10.1016/j.cmet.2024.07.024.

Phenotypic Characterization of Female Carrier Mice Heterozygous for Tafazzin Deletion.

Tomczewski M, Chan J, Al-Majmaie D, Liu M, Cocco A, Stark K Biology (Basel). 2023; 12(9).

PMID: 37759637 PMC: 10525480. DOI: 10.3390/biology12091238.

Genetic deletion of skeletal muscle iPLAγ results in mitochondrial dysfunction, muscle atrophy and alterations in whole-body energy metabolism.

Moon S, Dilthey B, Guan S, Sims H, Pittman S, Keith A iScience. 2023; 26(6):106895.

PMID: 37275531 PMC: 10239068. DOI: 10.1016/j.isci.2023.106895.

Separation and Simultaneous Trapping of Multiply Charged and Singly Charged Ions for Mass Spectrometry: Application to Lipid Mixtures.

Fabijanczuk K, Hager J, McLuckey S Anal Chem. 2023; 95(14):6115-6121.

PMID: 37000630 PMC: 10129443. DOI: 10.1021/acs.analchem.3c00420.

Novel Grade Classification Tool with Lipidomics for Indica Rice Eating Quality Evaluation.

Zhao L, Duan X, Liu H Foods. 2023; 12(5).

PMID: 36900461 PMC: 10000924. DOI: 10.3390/foods12050944.

References

McMillin J, Dowhan W . Cardiolipin and apoptosis. Biochim Biophys Acta. 2003; 1585(2-3):97-107. DOI: 10.1016/s1388-1981(02)00329-3. View

Schlame M, Towbin J, Heerdt P, Jehle R, DiMauro S, Blanck T . Deficiency of tetralinoleoyl-cardiolipin in Barth syndrome. Ann Neurol. 2002; 51(5):634-7. DOI: 10.1002/ana.10176. View

Cristea I, Degli Esposti M . Membrane lipids and cell death: an overview. Chem Phys Lipids. 2004; 129(2):133-60. DOI: 10.1016/j.chemphyslip.2004.02.002. View

Gross R . High plasmalogen and arachidonic acid content of canine myocardial sarcolemma: a fast atom bombardment mass spectroscopic and gas chromatography-mass spectroscopic characterization. Biochemistry. 1984; 23(1):158-65. DOI: 10.1021/bi00296a026. View

Gu Z, Valianpour F, Chen S, Vaz F, Hakkaart G, Wanders R . Aberrant cardiolipin metabolism in the yeast taz1 mutant: a model for Barth syndrome. Mol Microbiol. 2003; 51(1):149-58. DOI: 10.1046/j.1365-2958.2003.03802.x. View

Han X, Gross R . Shotgun lipidomics: electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples. Mass Spectrom Rev. 2004; 24(3):367-412. DOI: 10.1002/mas.20023. View

Han X, Cheng H, Mancuso D, Gross R . Caloric restriction results in phospholipid depletion, membrane remodeling, and triacylglycerol accumulation in murine myocardium. Biochemistry. 2004; 43(49):15584-94. DOI: 10.1021/bi048307o. View

Sparagna G, Johnson C, McCune S, Moore R, Murphy R . Quantitation of cardiolipin molecular species in spontaneously hypertensive heart failure rats using electrospray ionization mass spectrometry. J Lipid Res. 2005; 46(6):1196-204. DOI: 10.1194/jlr.M500031-JLR200. View

Han X, Yang J, Cheng H, Ye H, Gross R . Toward fingerprinting cellular lipidomes directly from biological samples by two-dimensional electrospray ionization mass spectrometry. Anal Biochem. 2004; 330(2):317-31. DOI: 10.1016/j.ab.2004.04.004. View

10.

Han X, Gross R . Shotgun lipidomics: multidimensional MS analysis of cellular lipidomes. Expert Rev Proteomics. 2005; 2(2):253-64. DOI: 10.1586/14789450.2.2.253. View

11.

Hsu F, Turk J, Rhoades E, Russell D, Shi Y, Groisman E . Structural characterization of cardiolipin by tandem quadrupole and multiple-stage quadrupole ion-trap mass spectrometry with electrospray ionization. J Am Soc Mass Spectrom. 2005; 16(4):491-504. DOI: 10.1016/j.jasms.2004.12.015. View

12.

Barth P, Valianpour F, Bowen V, Lam J, Duran M, Vaz F . X-linked cardioskeletal myopathy and neutropenia (Barth syndrome): an update. Am J Med Genet A. 2004; 126A(4):349-54. DOI: 10.1002/ajmg.a.20660. View

13.

Schlame M, Rua D, Greenberg M . The biosynthesis and functional role of cardiolipin. Prog Lipid Res. 2000; 39(3):257-88. DOI: 10.1016/s0163-7827(00)00005-9. View

14.

Schlame M, Ren M, Xu Y, Greenberg M, Haller I . Molecular symmetry in mitochondrial cardiolipins. Chem Phys Lipids. 2005; 138(1-2):38-49. DOI: 10.1016/j.chemphyslip.2005.08.002. View

15.

Valianpour F, Wanders R, Overmars H, Vreken P, van Gennip A, Baas F . Cardiolipin deficiency in X-linked cardioskeletal myopathy and neutropenia (Barth syndrome, MIM 302060): a study in cultured skin fibroblasts. J Pediatr. 2002; 141(5):729-33. DOI: 10.1067/mpd.2002.129174. View

16.

Han X, Gross R . Quantitative analysis and molecular species fingerprinting of triacylglyceride molecular species directly from lipid extracts of biological samples by electrospray ionization tandem mass spectrometry. Anal Biochem. 2001; 295(1):88-100. DOI: 10.1006/abio.2001.5178. View

17.

Han X, Yang K, Cheng H, Fikes K, Gross R . Shotgun lipidomics of phosphoethanolamine-containing lipids in biological samples after one-step in situ derivatization. J Lipid Res. 2005; 46(7):1548-60. PMC: 2141546. DOI: 10.1194/jlr.D500007-JLR200. View

18.

Han X, Yang J, Cheng H, Yang K, Abendschein D, Gross R . Shotgun lipidomics identifies cardiolipin depletion in diabetic myocardium linking altered substrate utilization with mitochondrial dysfunction. Biochemistry. 2005; 44(50):16684-94. DOI: 10.1021/bi051908a. View

19.

Teng J, Smith L . High-performance liquid chromatography of cardiolipin. J Chromatogr. 1985; 339(1):35-44. DOI: 10.1016/s0378-4347(00)84625-3. View

20.

BLIGH E, Dyer W . A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-7. DOI: 10.1139/o59-099. View