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» Articles » PMID: 16395626

Quantification of Zeaxanthin Dipalmitate and Total Carotenoids in Lycium Fruits (Fructus Lycii)

Overview
Journal Plant Foods Hum Nutr
Specialties Biology
Nutritional Sciences
Date 2006 Jan 6
PMID 16395626
Citations 30
Authors
Yong Peng
Chen Ma
Yawei Li
Kelvin Sze-Yin Leung
Zhi-Hong Jiang
Zhongzhen Zhao
Affiliations
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Abstract

An HPLC-DAD method has been developed to quantitatively analyze for the content of zeaxanthin dipalmitate, a major carotenoid in Fructus Lycii, in different species of the genus Lycium. Determination was performed using an Alltima C18 column with the mobile phase consisting of acetonitrile and dichloromethane (42:58). The total contents of carotenoids in these samples were also determined by using UV spectrophotometric assay. Total carotenoid concentrations of different Fructus Lycii are within the range of 0.03-0.5%. Zeaxanthin dipalmitate is a predominant carotenoid, comprising 31-56% of the total carotenoids in Fructus Lycii. This study is the first systematic quantification of the carotenoids in the fruits of different Lycium species. The results demonstrated that these methods are reliable and facile techniques for rapid analysis of carotenoids for crude drug and plant-derived food supplements.

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