Catalyzed Reporter Deposition-fluorescence in Situ Hybridization Allows for Enrichment-independent Detection of Microcolony-forming Soil Bacteria

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2006 Jan 5

PMID 16391135

Citations 9

Authors

Belinda C Ferrari

Niina Tujula

Kate Stoner

Staffan Kjelleberg

Affiliations

Soon will be listed here.

Abstract

Advances in the growth of hitherto unculturable soil bacteria have emphasized the requirement for rapid bacterial identification methods. Due to the slow-growing strategy of microcolony-forming soil bacteria, successful fluorescence in situ hybridization (FISH) requires an rRNA enrichment step for visualization. In this study, catalyzed reporter deposition (CARD)-FISH was employed as an alternative method to rRNA enhancement and was found to be superior to conventional FISH for the detection of microcolonies that are cultivated by using the soil substrate membrane system. CARD-FISH enabled real-time identification of oligophilic microcolony-forming soil bacteria without the requirement for enrichment on complex media and the associated shifts in community composition.

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