Cultivation of Fastidious Bacteria by Viability Staining and Micromanipulation in a Soil Substrate Membrane System

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2009 Mar 24

PMID 19304839

Citations 14

Authors

B C Ferrari

M R Gillings

Affiliations

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Abstract

Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.

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