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Multiplex PCR Assay That Identifies the Major Lipooligosaccharide Serotype Expressed by Moraxella Catarrhalis Clinical Isolates

Overview
Specialty Microbiology
Date 2005 Dec 8
PMID 16333114
Citations 10
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Abstract

A heterologous cluster of glycosyltransferase genes was identified in the three Moraxella catarrhalis LOS serotype strains. Multiple PCR primers designed to this region amplified products that differentiate between the serotypes more rapidly and efficiently than previously described serological analyses. This assay will be valuable for clinical and research-based studies.

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References
1.
Holme T, Rahman M, Jansson P, Widmalm G . The lipopolysaccharide of moraxella catarrhalis structural relationships and antigenic properties. Eur J Biochem. 1999; 265(2):524-9. DOI: 10.1046/j.1432-1327.1999.00731.x. View

2.
Murphy T, Kirkham C, DeNardin E, Sethi S . Analysis of antigenic structure and human immune response to outer membrane protein CD of Moraxella catarrhalis. Infect Immun. 1999; 67(9):4578-85. PMC: 96781. DOI: 10.1128/IAI.67.9.4578-4585.1999. View

3.
Edwards K, Allen S, Gibson B, Campagnari A . Characterization of a cluster of three glycosyltransferase enzymes essential for Moraxella catarrhalis lipooligosaccharide assembly. J Bacteriol. 2005; 187(9):2939-47. PMC: 1082826. DOI: 10.1128/JB.187.9.2939-2947.2005. View

4.
Peng D, Choudhury B, Petralia R, Carlson R, Gu X . Roles of 3-deoxy-D-manno-2-octulosonic acid transferase from Moraxella catarrhalis in lipooligosaccharide biosynthesis and virulence. Infect Immun. 2005; 73(7):4222-30. PMC: 1168618. DOI: 10.1128/IAI.73.7.4222-4230.2005. View

5.
Murphy T, Brauer A, Grant B, Sethi S . Moraxella catarrhalis in chronic obstructive pulmonary disease: burden of disease and immune response. Am J Respir Crit Care Med. 2005; 172(2):195-9. PMC: 2718466. DOI: 10.1164/rccm.200412-1747OC. View