The Moraxella Catarrhalis Porin-like Outer Membrane Protein CD is an Adhesin for Human Lung Cells

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2004 Mar 25

PMID 15039309

Citations 45

Authors

Melissa M Holm

Serena L Vanlerberg

Ian M Foley

Darren D Sledjeski

Eric R Lafontaine

Affiliations

Soon will be listed here.

Abstract

The outer membrane protein CD (OMPCD) of Moraxella catarrhalis is an outer membrane protein with several attributes of a potential vaccine antigen. We isolated four transposon mutants of strain O35E on the basis of their reduced binding to A549 human lung cells in microcolony formation assays, and we determined that they contain a transposon in ompCD. We also found that these transposon insertions had pleiotropic effects: mutants grew slower, became serum sensitive, bound approximately 10-fold less to A549 cells, and appeared transparent when grown on solid medium. We confirmed that these various phenotypes could be attributed solely to disruption of ompCD by constructing the isogenic strain O35E.CD1. O35E-ompCD was cloned, and recombinant Escherichia coli bacteria expressing the gene product exhibited a 10-fold increase in adherence to A549 cells. This is the first report of M. catarrhalis ompCD mutants, and our findings demonstrate that this gene product is an adhesin for human lung cells.

Citing Articles

The Direct Anti-Virulence but Not Bactericidal Activity of Human Neutrophil Elastase against .

Roszkowiak J, McClean S, Mironczuk A, Augustyniak D Int J Mol Sci. 2023; 24(7).

PMID: 37047578 PMC: 10094786. DOI: 10.3390/ijms24076607.

Genotypic and Phenotypic Characteristics of from Patients and Healthy Asymptomatic Participants among Preschool Children.

Zhao N, Ren H, Deng J, Du Y, Li Q, Zhou P Pathogens. 2022; 11(9).

PMID: 36145417 PMC: 9503219. DOI: 10.3390/pathogens11090984.

Stringently Defined Otitis Prone Children Demonstrate Deficient Naturally Induced Mucosal Antibody Response to Proteins.

Ren D, Murphy T, Lafontaine E, E Pichichero M Front Immunol. 2017; 8:953.

PMID: 28848555 PMC: 5554491. DOI: 10.3389/fimmu.2017.00953.

Vaccine targets against Moraxella catarrhalis.

Ren D, E Pichichero M Expert Opin Ther Targets. 2015; 20(1):19-33.

PMID: 26565427 PMC: 4720561. DOI: 10.1517/14728222.2015.1081686.

Participation of the Salmonella OmpD porin in the infection of RAW264.7 macrophages and BALB/c mice.

Ipinza F, Collao B, Monsalva D, Bustamante V, Luraschi R, Alegria-Arcos M PLoS One. 2014; 9(10):e111062.

PMID: 25360745 PMC: 4215857. DOI: 10.1371/journal.pone.0111062.

References

Forsgren A, Brant M, Karamehmedovic M, Riesbeck K . The immunoglobulin D-binding protein MID from Moraxella catarrhalis is also an adhesin. Infect Immun. 2003; 71(6):3302-9. PMC: 155714. DOI: 10.1128/IAI.71.6.3302-3309.2003. View

Timpe J, Holm M, Vanlerberg S, Basrur V, Lafontaine E . Identification of a Moraxella catarrhalis outer membrane protein exhibiting both adhesin and lipolytic activities. Infect Immun. 2003; 71(8):4341-50. PMC: 166007. DOI: 10.1128/IAI.71.8.4341-4350.2003. View

Holm M, Vanlerberg S, Sledjeski D, Lafontaine E . The Hag protein of Moraxella catarrhalis strain O35E is associated with adherence to human lung and middle ear cells. Infect Immun. 2003; 71(9):4977-84. PMC: 187358. DOI: 10.1128/IAI.71.9.4977-4984.2003. View

Utsunomiya T, Nakahara K, Kuramochi M, Hashiba K, Uzuka Y, Matsumoto K . [Branhamella (Neisseria) catarrhalis endocarditis after insertion of a mitral prosthesis: a case report]. Nihon Naika Gakkai Zasshi. 1984; 73(10):1506-11. DOI: 10.2169/naika.73.1506. View

Turner H, Taylor M, LOCKWOOD W . Branhamella catarrhalis endocarditis in a patient receiving hemodialysis. South Med J. 1985; 78(8):1021-2. DOI: 10.1097/00007611-198508000-00041. View

Patrick C, Kimura A, Jackson M, Hermanstorfer L, Hood A, McCracken Jr G . Antigenic characterization of the oligosaccharide portion of the lipooligosaccharide of nontypable Haemophilus influenzae. Infect Immun. 1987; 55(12):2902-11. PMC: 260004. DOI: 10.1128/iai.55.12.2902-2911.1987. View

Murphy T, Loeb M . Isolation of the outer membrane of Branhamella catarrhalis. Microb Pathog. 1989; 6(3):159-74. DOI: 10.1016/0882-4010(89)90066-1. View

CATLIN B . Branhamella catarrhalis: an organism gaining respect as a pathogen. Clin Microbiol Rev. 1990; 3(4):293-320. PMC: 358165. DOI: 10.1128/CMR.3.4.293. View

Weiser J, Gotschlich E . Outer membrane protein A (OmpA) contributes to serum resistance and pathogenicity of Escherichia coli K-1. Infect Immun. 1991; 59(7):2252-8. PMC: 258003. DOI: 10.1128/iai.59.7.2252-2258.1991. View

10.

Sethi S, Evans N, Grant B, Murphy T . New strains of bacteria and exacerbations of chronic obstructive pulmonary disease. N Engl J Med. 2002; 347(7):465-71. DOI: 10.1056/NEJMoa012561. View

11.

Azghani A, Idell S, Bains M, Hancock R . Pseudomonas aeruginosa outer membrane protein F is an adhesin in bacterial binding to lung epithelial cells in culture. Microb Pathog. 2002; 33(3):109-14. DOI: 10.1006/mpat.2002.0514. View

12.

Chen D, Barniak V, VanDerMeid K, McMichael J . The levels and bactericidal capacity of antibodies directed against the UspA1 and UspA2 outer membrane proteins of Moraxella (Branhamella) catarrhalis in adults and children. Infect Immun. 1999; 67(3):1310-6. PMC: 96462. DOI: 10.1128/IAI.67.3.1310-1316.1999. View

13.

Fitzgerald M, Mulcahy R, Murphy S, Keane C, Coakley D, Scott T . Transmission electron microscopy studies of Moraxella (Branhamella) catarrhalis. FEMS Immunol Med Microbiol. 1999; 23(1):57-66. DOI: 10.1111/j.1574-695X.1999.tb01717.x. View

14.

Klebba P, Newton S . Mechanisms of solute transport through outer membrane porins: burning down the house. Curr Opin Microbiol. 1999; 1(2):238-47. DOI: 10.1016/s1369-5274(98)80017-9. View

15.

Neumayer U, Schmidt H, Mellwig K, Kleikamp G . Moraxella catarrhalis endocarditis: report of a case and literature review. J Heart Valve Dis. 1999; 8(1):114-7. View

16.

Christensen J . Moraxella (Branhamella) catarrhalis: clinical, microbiological and immunological features in lower respiratory tract infections. APMIS Suppl. 1999; 88:1-36. View

17.

Cope L, Lafontaine E, Slaughter C, Hasemann Jr C, Aebi C, Henderson F . Characterization of the Moraxella catarrhalis uspA1 and uspA2 genes and their encoded products. J Bacteriol. 1999; 181(13):4026-34. PMC: 93893. DOI: 10.1128/JB.181.13.4026-4034.1999. View

18.

Yu R, Bonnah R, Ainsworth S, Schryvers A . Analysis of the immunological responses to transferrin and lactoferrin receptor proteins from Moraxella catarrhalis. Infect Immun. 1999; 67(8):3793-9. PMC: 96656. DOI: 10.1128/IAI.67.8.3793-3799.1999. View

19.

Murphy T, Kirkham C, DeNardin E, Sethi S . Analysis of antigenic structure and human immune response to outer membrane protein CD of Moraxella catarrhalis. Infect Immun. 1999; 67(9):4578-85. PMC: 96781. DOI: 10.1128/IAI.67.9.4578-4585.1999. View

20.

Sarwar J, Campagnari A, Kirkham C, Murphy T . Characterization of an antigenically conserved heat-modifiable major outer membrane protein of Branhamella catarrhalis. Infect Immun. 1992; 60(3):804-9. PMC: 257558. DOI: 10.1128/iai.60.3.804-809.1992. View