Use of Next Generation Sequence to Investigate Potential Novel Macrolide Resistance Mechanisms in a Population of Moraxella Catarrhalis Isolates

Overview

Journal Sci Rep

Specialty Science

Date 2016 Oct 25

PMID 27774989

Citations 4

Authors

Ya-Li Liu

Dong-Fang Li

He-Ping Xu

Meng Xiao

Jing-Wei Cheng

Li Zhang

Zhi-Peng Xu

Xin-Xin Chen

Ge Zhang

Timothy Kudinha

Fanrong Kong

Yan-Ping Gong

Xin-Ying Wang

Yin-Xin Zhang

Hong-Long Wu

Ying-Chun Xu

Affiliations

Soon will be listed here.

Abstract

Although previous studies have confirmed that 23S rRNA gene mutation could be responsible for most of macrolide resistance in M. catarrhalis, a recent study suggested otherwise. Next generation sequence based comparative genomics has revolutionized the mining of potential novel drug resistant mechanisms. In this study, two pairs of resistant and susceptible M. catarrhalis isolates with different multilocus sequence types, were investigated for potential differential genes or informative single nucleotide polymorphisms (SNPs). The identified genes and SNPs were evaluated in 188 clinical isolates. From initially 12 selected differential genes and 12 informative SNPs, 10 differential genes (mboIA, mcbC, mcbI, mboIB, MCR_1794, MCR_1795, lgt2B/C, dpnI, mcbB, and mcbA) and 6 SNPs (C619T of rumA, T140C of rplF, G643A of MCR_0020, T270G of MCR_1465, C1348A of copB, and G238A of rrmA) were identified as possibly linked to macrolide resistance in M. catarrhalis. Most of the identified differential genes and SNPs are related to methylation of ribosomal RNA (rRNA) or DNA, especially MCR_0020 and rrmA. Further studies are needed to determine the function and/or evolution process, of the identified genes or SNPs, to establish whether some novel or combined mechanisms are truly involved in M. catarrhalis macrolide resistance mechanism.

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