Shiga Toxin 2e-producing Escherichia Coli Isolates from Humans and Pigs Differ in Their Virulence Profiles and Interactions with Intestinal Epithelial Cells

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2005 Dec 8

PMID 16332882

Citations 47

Authors

Anne-Katharina Sonntag

Martina Bielaszewska

Alexander Mellmann

Nadine Dierksen

Peter Schierack

Lothar H Wieler

M Alexander Schmidt

Helge Karch

Affiliations

Soon will be listed here.

Abstract

Thirteen Escherichia coli strains harboring stx2e were isolated from 11,056 human stools. This frequency corresponded to the presence of the stx2e allele in 1.7% of all Shiga toxin-producing E. coli (STEC) strains. The strains harboring stx2e were associated with mild diarrhea (n = 9) or asymptomatic infections (n = 4). Because STEC isolates possessing stx2e are porcine pathogens, we compared the human STEC isolates with stx2e-harboring E. coli isolated from piglets with edema disease and postweaning diarrhea. All pig isolates possessed the gene encoding the F18 adhesin, and the majority possessed adhesin involved in diffuse adherence; these adhesins were absent from all the human STEC isolates. In contrast, the high-pathogenicity island encoding an iron uptake system was found only in human isolates. Host-specific patterns of interaction with intestinal epithelial cells were observed. All human isolates adhered to human intestinal epithelial cell lines T84 and HCT-8 but not to pig intestinal epithelial cell line IPEC-J2. In contrast, the pig isolates completely lysed human epithelial cells but not IPEC-J2 cells, to which most of them adhered. Our data demonstrate that E. coli isolates producing Shiga toxin 2e have imported specific virulence and fitness determinants which allow them to adapt to the specific hosts in which they cause various forms of disease.

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References

Schubert S, Picard B, Gouriou S, Heesemann J, Denamur E . Yersinia high-pathogenicity island contributes to virulence in Escherichia coli causing extraintestinal infections. Infect Immun. 2002; 70(9):5335-7. PMC: 128248. DOI: 10.1128/IAI.70.9.5335-5337.2002. View

Friedrich A, Kock R, Bielaszewska M, Zhang W, Karch H, Mathys W . Distribution of the urease gene cluster among and urease activities of enterohemorrhagic Escherichia coli O157 isolates from humans. J Clin Microbiol. 2005; 43(2):546-50. PMC: 548110. DOI: 10.1128/JCM.43.2.546-550.2005. View

Tarr P, Gordon C, Chandler W . Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome. Lancet. 2005; 365(9464):1073-86. DOI: 10.1016/S0140-6736(05)71144-2. View

Bielaszewska M, Zhang W, Tarr P, Sonntag A, Karch H . Molecular profiling and phenotype analysis of Escherichia coli O26:H11 and O26:NM: secular and geographic consistency of enterohemorrhagic and enteropathogenic isolates. J Clin Microbiol. 2005; 43(8):4225-8. PMC: 1233958. DOI: 10.1128/JCM.43.8.4225-4228.2005. View

Janka A, Becker G, Sonntag A, Bielaszewska M, Dobrindt U, Karch H . Presence and characterization of a mosaic genomic island which distinguishes sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H- from E. coli O157:H7. Appl Environ Microbiol. 2005; 71(8):4875-8. PMC: 1183287. DOI: 10.1128/AEM.71.8.4875-4878.2005. View

Salvadori M, Yamada A, Yano T . Morphological and intracellular alterations induced by cytotoxin VT2y produced by Escherichia coli isolated from chickens with swollen head syndrome. FEMS Microbiol Lett. 2001; 197(1):79-84. PMC: 7110317. DOI: 10.1111/j.1574-6968.2001.tb10586.x. View

Nicholls L, Grant T, Robins-Browne R . Identification of a novel genetic locus that is required for in vitro adhesion of a clinical isolate of enterohaemorrhagic Escherichia coli to epithelial cells. Mol Microbiol. 2000; 35(2):275-88. DOI: 10.1046/j.1365-2958.2000.01690.x. View

Tarr P, Bilge S, Vary Jr J, Jelacic S, Habeeb R, Ward T . Iha: a novel Escherichia coli O157:H7 adherence-conferring molecule encoded on a recently acquired chromosomal island of conserved structure. Infect Immun. 2000; 68(3):1400-7. PMC: 97294. DOI: 10.1128/IAI.68.3.1400-1407.2000. View

Zhang W, Bielaszewska M, Liesegang A, Tschape H, Schmidt H, Bitzan M . Molecular characteristics and epidemiological significance of Shiga toxin-producing Escherichia coli O26 strains. J Clin Microbiol. 2000; 38(6):2134-40. PMC: 86746. DOI: 10.1128/JCM.38.6.2134-2140.2000. View

10.

Niewerth U, Frey A, Voss T, Le Bouguenec C, Baljer G, Franke S . The AIDA autotransporter system is associated with F18 and stx2e in Escherichia coli isolates from pigs diagnosed with edema disease and postweaning diarrhea. Clin Diagn Lab Immunol. 2001; 8(1):143-9. PMC: 96024. DOI: 10.1128/CDLI.8.1.143-149.2001. View

11.

Morabito S, DellOmo G, Agrimi U, Schmidt H, Karch H, Cheasty T . Detection and characterization of Shiga toxin-producing Escherichia coli in feral pigeons. Vet Microbiol. 2001; 82(3):275-83. DOI: 10.1016/s0378-1135(01)00393-5. View

12.

Schmidt H, Zhang W, Hemmrich U, Jelacic S, Brunder W, Tarr P . Identification and characterization of a novel genomic island integrated at selC in locus of enterocyte effacement-negative, Shiga toxin-producing Escherichia coli. Infect Immun. 2001; 69(11):6863-73. PMC: 100065. DOI: 10.1128/IAI.69.11.6863-6873.2001. View

13.

Paton A, Srimanote P, Woodrow M, Paton J . Characterization of Saa, a novel autoagglutinating adhesin produced by locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli strains that are virulent for humans. Infect Immun. 2001; 69(11):6999-7009. PMC: 100080. DOI: 10.1128/IAI.69.11.6999-7009.2001. View

14.

Friedrich A, Bielaszewska M, Zhang W, Pulz M, Kuczius T, Ammon A . Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptoms. J Infect Dis. 2002; 185(1):74-84. DOI: 10.1086/338115. View

15.

Nakano M, Iida T, Ohnishi M, Kurokawa K, Takahashi A, Tsukamoto T . Association of the urease gene with enterohemorrhagic Escherichia coli strains irrespective of their serogroups. J Clin Microbiol. 2001; 39(12):4541-3. PMC: 88583. DOI: 10.1128/JCM.39.12.4541-4543.2001. View

16.

Zhang W, Bielaszewska M, Kuczius T, Karch H . Identification, characterization, and distribution of a Shiga toxin 1 gene variant (stx(1c)) in Escherichia coli strains isolated from humans. J Clin Microbiol. 2002; 40(4):1441-6. PMC: 140390. DOI: 10.1128/JCM.40.4.1441-1446.2002. View

17.

Lathem W, Grys T, Witowski S, Torres A, Kaper J, Tarr P . StcE, a metalloprotease secreted by Escherichia coli O157:H7, specifically cleaves C1 esterase inhibitor. Mol Microbiol. 2002; 45(2):277-88. DOI: 10.1046/j.1365-2958.2002.02997.x. View

18.

Taylor D, Rooker M, Keelan M, Ng L, Martin I, Perna N . Genomic variability of O islands encoding tellurite resistance in enterohemorrhagic Escherichia coli O157:H7 isolates. J Bacteriol. 2002; 184(17):4690-8. PMC: 135296. DOI: 10.1128/JB.184.17.4690-4698.2002. View

19.

Gerber A, Karch H, Allerberger F, Verweyen H, Zimmerhackl L . Clinical course and the role of shiga toxin-producing Escherichia coli infection in the hemolytic-uremic syndrome in pediatric patients, 1997-2000, in Germany and Austria: a prospective study. J Infect Dis. 2002; 186(4):493-500. DOI: 10.1086/341940. View

20.

Janka A, Bielaszewska M, Dobrindt U, Karch H . Identification and distribution of the enterohemorrhagic Escherichia coli factor for adherence (efa1) gene in sorbitol-fermenting Escherichia coli O157: H-. Int J Med Microbiol. 2002; 292(3-4):207-14. DOI: 10.1078/1438-4221-00206. View