Olive Mill Waste-Water Extract Enriched in Hydroxytyrosol and Tyrosol Modulates Host-Pathogen Interaction in IPEC-J2 Cells

Overview

Journal Animals (Basel)

Date 2024 Feb 24

PMID 38396532

Authors

Flavia Ferlisi

Chiara Grazia De Ciucis

Massimo Trabalza-Marinucci

Floriana Fruscione

Samanta Mecocci

Giulia Franzoni

Susanna Zinellu

Roberta Galarini

Elisabetta Razzuoli

Katia Cappelli

Affiliations

Soon will be listed here.

Abstract

The dietary supplementation of olive oil by-products, including olive mill waste-water (OMWW) in animal diets, is a novel application that allows for their re-utilization and recycling and could potentially decrease the use of antibiotics, antimicrobial resistance risk in livestock species, and the occurrence of intestinal diseases. is one of the most widespread intestinal pathogens in the world, causing enterocolitis in pigs. The aim of this study was to investigate the effect of an OMWW extract enriched in polyphenols (hydroxytyrosol and tyrosol) in the immune response of an intestinal porcine epithelial cell line (IPEC-J2) following infection. Cells were pre-treated with OMWW-extract polyphenols (OMWW-EP, 0.35 and 1.4 µg) for 24 h and then infected with for 1 h. We evaluated bacterial invasiveness and assayed IPEC-J2 gene expression with RT-qPCR and cytokine release with an ELISA test. The obtained results showed that OMWW-EP (1.4 µg) significantly reduced invasiveness; 0.35 µg decreased the IPEC-J2 gene expression of , , and , while 1.4 µg down-regulated and and increased The cytokine content was unchanged in infected cells. This is the first study demonstrating the immunomodulatory and antimicrobial activity of OMWW extracts enriched in polyphenols, suggesting a protective role of OMWW polyphenols on the pig intestine and their potential application as feed supplements in farm animals such as pigs.

Citing Articles

Therapeutic potential of plant polyphenols in acute pancreatitis.

Niu C, Zhang J, Okolo 3rd P Inflammopharmacology. 2024; 33(2):785-798.

PMID: 39497005 DOI: 10.1007/s10787-024-01584-y.

References

Gonzalez R, Ballester I, Lopez-Posadas R, Suarez M, Zarzuelo A, Martinez-Augustin O . Effects of flavonoids and other polyphenols on inflammation. Crit Rev Food Sci Nutr. 2011; 51(4):331-62. DOI: 10.1080/10408390903584094. View

Serrano-Lopez J, Martin-Antonio B . Inflammaging, an Imbalanced Immune Response That Needs to Be Restored for Cancer Prevention and Treatment in the Elderly. Cells. 2021; 10(10). PMC: 8533838. DOI: 10.3390/cells10102562. View

Dinarello C . Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 2017; 281(1):8-27. PMC: 5756628. DOI: 10.1111/imr.12621. View

Ling K, Wan M, El-Nezami H, Wang M . Protective Capacity of Resveratrol, a Natural Polyphenolic Compound, against Deoxynivalenol-Induced Intestinal Barrier Dysfunction and Bacterial Translocation. Chem Res Toxicol. 2016; 29(5):823-33. DOI: 10.1021/acs.chemrestox.6b00001. View

Qin G, Zhao Y, Gan Y, Yu X, Zhao Y, Peng H . Alterations in gene expressions of Caco-2 cell responses to LPS and ploy(I:C) stimulation. PeerJ. 2023; 11:e15459. PMC: 10257391. DOI: 10.7717/peerj.15459. View

Veldhuizen E, Koomen I, Ultee T, van Dijk A, Haagsman H . Salmonella serovar specific upregulation of porcine defensins 1 and 2 in a jejunal epithelial cell line. Vet Microbiol. 2008; 136(1-2):69-75. DOI: 10.1016/j.vetmic.2008.09.072. View

Ahasan A, Invernizzi G, Farina G, Pilotto A, Barbe F, Bontempo V . The effects of superoxide dismutase-rich melon pulp concentrate on inflammation, antioxidant status and growth performance of challenged post-weaning piglets. Animal. 2018; 13(1):136-143. DOI: 10.1017/S1751731118001234. View

Mecocci S, De Paolis L, Zoccola R, Fruscione F, De Ciucis C, Chiaradia E . Antimicrobial and Immunomodulatory Potential of Cow Colostrum Extracellular Vesicles (ColosEVs) in an Intestinal In Vitro Model. Biomedicines. 2022; 10(12). PMC: 9775086. DOI: 10.3390/biomedicines10123264. View

Salh B, Assi K, Templeman V, Parhar K, Owen D, Gomez-Munoz A . Curcumin attenuates DNB-induced murine colitis. Am J Physiol Gastrointest Liver Physiol. 2003; 285(1):G235-43. DOI: 10.1152/ajpgi.00449.2002. View

10.

Razzuoli E, Villa R, Amadori M . IPEC-J2 cells as reporter system of the anti-inflammatory control actions of interferon-alpha. J Interferon Cytokine Res. 2013; 33(10):597-605. PMC: 3793657. DOI: 10.1089/jir.2012.0127. View

11.

Naberhaus S, Krull A, Arruda B, Arruda P, Sahin O, Schwartz K . Pathogenicity and Competitive Fitness of Serovar 4,[5],12:i:- Compared to Typhimurium and Derby in Swine. Front Vet Sci. 2020; 6:502. PMC: 7002397. DOI: 10.3389/fvets.2019.00502. View

12.

Razzuoli E, Vencia W, Modesto P, Franzoni G, Dei Giudici S, Parisi E . Yersinia enterocolitica-specific modulation of innate immune responses in jejunal epithelial cells. Vet Microbiol. 2020; 242:108596. DOI: 10.1016/j.vetmic.2020.108596. View

13.

Mekki A, Dhouib A, Sayadi S . Changes in microbial and soil properties following amendment with treated and untreated olive mill wastewater. Microbiol Res. 2006; 161(2):93-101. DOI: 10.1016/j.micres.2005.06.001. View

14.

Sahoo M, Ceballos-Olvera I, Del Barrio L, Re F . Role of the inflammasome, IL-1β, and IL-18 in bacterial infections. ScientificWorldJournal. 2011; 11:2037-50. PMC: 3217589. DOI: 10.1100/2011/212680. View

15.

Mecocci S, De Paolis L, Fruscione F, Pietrucci D, De Ciucis C, Dei Giudici S . In vitro evaluation of immunomodulatory activities of goat milk Extracellular Vesicles (mEVs) in a model of gut inflammation. Res Vet Sci. 2022; 152:546-556. DOI: 10.1016/j.rvsc.2022.09.021. View

16.

Quaratino D, DAnnibale A, Federici F, Cereti C, Rossini F, Fenice M . Enzyme and fungal treatments and a combination thereof reduce olive mill wastewater phytotoxicity on Zea mays L. seeds. Chemosphere. 2006; 66(9):1627-33. DOI: 10.1016/j.chemosphere.2006.07.092. View

17.

Gessner D, Ringseis R, Siebers M, Keller J, Kloster J, Wen G . Inhibition of the pro-inflammatory NF-κB pathway by a grape seed and grape marc meal extract in intestinal epithelial cells. J Anim Physiol Anim Nutr (Berl). 2011; 96(6):1074-83. DOI: 10.1111/j.1439-0396.2011.01222.x. View

18.

Ferlisi F, Tang J, Cappelli K, Trabalza-Marinucci M . Dietary supplementation with olive oil co-products rich in polyphenols: a novel nutraceutical approach in monogastric animal nutrition. Front Vet Sci. 2023; 10:1272274. PMC: 10611480. DOI: 10.3389/fvets.2023.1272274. View

19.

van den Bogaard A, Stobberingh E . Antibiotic usage in animals: impact on bacterial resistance and public health. Drugs. 1999; 58(4):589-607. DOI: 10.2165/00003495-199958040-00002. View

20.

Dejban P, Nikravangolsefid N, Chamanara M, Dehpour A, Rashidian A . The role of medicinal products in the treatment of inflammatory bowel diseases (IBD) through inhibition of TLR4/NF-kappaB pathway. Phytother Res. 2020; 35(2):835-845. DOI: 10.1002/ptr.6866. View