Characterization of a Mis12 Homologue in Arabidopsis Thaliana

Overview

Journal Chromosome Res

Date 2005 Dec 7

PMID 16331414

Citations 23

Authors

Hiroshi Sato

Fukashi Shibata

Minoru Murata

Affiliations

Soon will be listed here.

Abstract

The centromere/kinetochore represents an important complex on chromosomes that contains a large number of proteins and facilitates accurate chromosome segregation during cell division. Fission yeast Mis12 and its human homologue hMis12 have been identified as essential kinetochore components. Although homologues have been suggested to exist in plants, their function remains to be determined. In this study the full-length cDNA of the Mis12 homologue from Arabidopsis thaliana (AtMIS12) was successfully cloned by RACE-and RT-PCR and the DNA sequence determined. The 238 amino acid sequence deduced from the cDNA contains two conserved blocks and a coiled-coil motif, despite the poor overall similarity to fission yeast and human Mis12. The antibody raised against a partial peptide of AtMIS12 recognized a 27-kDa protein corresponding to the predicted molecular weight. Immunofluorescence labeling using the antibody revealed that AtMIS12 localizes at centromeric regions, like the centromeric histone H3 variant HTR12, throughout the cell cycle. These results indicate that AtMIS12 is a constitutive component of Arabidopsis kinetochores.

Citing Articles

Recent advances in plant kinetochore research.

Kozgunova E Front Cell Dev Biol. 2025; 12:1510019.

PMID: 39911184 PMC: 11794483. DOI: 10.3389/fcell.2024.1510019.

Plant kinetochore complex: composition, function, and regulation.

Xie Y, Wang M, Mo B, Liang C Front Plant Sci. 2024; 15:1467236.

PMID: 39464281 PMC: 11503545. DOI: 10.3389/fpls.2024.1467236.

Holocentromeres can consist of merely a few megabase-sized satellite arrays.

Kuo Y, Camara A, Schubert V, Neumann P, Macas J, Melzer M Nat Commun. 2023; 14(1):3502.

PMID: 37311740 PMC: 10264360. DOI: 10.1038/s41467-023-38922-7.

Engineering apomixis in crops.

Mahlandt A, Singh D, Mercier R Theor Appl Genet. 2023; 136(6):131.

PMID: 37199785 PMC: 10195744. DOI: 10.1007/s00122-023-04357-3.

A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis.

Capitao C, Tanasa S, Fulnecek J, Raxwal V, Akimcheva S, Bulankova P PLoS Genet. 2021; 17(9):e1009779.

PMID: 34591845 PMC: 8509889. DOI: 10.1371/journal.pgen.1009779.

References

Howman E, Fowler K, Newson A, Redward S, Macdonald A, Kalitsis P . Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice. Proc Natl Acad Sci U S A. 2000; 97(3):1148-53. PMC: 15551. DOI: 10.1073/pnas.97.3.1148. View

Goshima G, Saitoh S, Yanagida M . Proper metaphase spindle length is determined by centromere proteins Mis12 and Mis6 required for faithful chromosome segregation. Genes Dev. 1999; 13(13):1664-77. PMC: 316848. DOI: 10.1101/gad.13.13.1664. View

Fukagawa T, Mikami Y, Nishihashi A, Regnier V, Haraguchi T, Hiraoka Y . CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells. EMBO J. 2001; 20(16):4603-17. PMC: 125570. DOI: 10.1093/emboj/20.16.4603. View

Shibata F, Murata M . Differential localization of the centromere-specific proteins in the major centromeric satellite of Arabidopsis thaliana. J Cell Sci. 2004; 117(Pt 14):2963-70. DOI: 10.1242/jcs.01144. View

Dawe R, Reed L, Yu H, Muszynski M, Hiatt E . A maize homolog of mammalian CENPC is a constitutive component of the inner kinetochore. Plant Cell. 1999; 11(7):1227-38. PMC: 144275. DOI: 10.1105/tpc.11.7.1227. View

Goshima G, Kiyomitsu T, Yoda K, Yanagida M . Human centromere chromatin protein hMis12, essential for equal segregation, is independent of CENP-A loading pathway. J Cell Biol. 2003; 160(1):25-39. PMC: 2172742. DOI: 10.1083/jcb.200210005. View

Mathur J, Szabados L, Schaefer S, Grunenberg B, Lossow A, Schell J . Gene identification with sequenced T-DNA tags generated by transformation of Arabidopsis cell suspension. Plant J. 1998; 13(5):707-16. DOI: 10.1046/j.1365-313x.1998.00059.x. View

Ogura Y, Shibata F, Sato H, Murata M . Characterization of a CENP-C homolog in Arabidopsis thaliana. Genes Genet Syst. 2004; 79(3):139-44. DOI: 10.1266/ggs.79.139. View

Ahmad K, Henikoff S . Histone H3 variants specify modes of chromatin assembly. Proc Natl Acad Sci U S A. 2002; 99 Suppl 4:16477-84. PMC: 139911. DOI: 10.1073/pnas.172403699. View

10.

Fukagawa T, Pendon C, Morris J, Brown W . CENP-C is necessary but not sufficient to induce formation of a functional centromere. EMBO J. 1999; 18(15):4196-209. PMC: 1171496. DOI: 10.1093/emboj/18.15.4196. View

11.

Nishihashi A, Haraguchi T, Hiraoka Y, Ikemura T, Regnier V, Dodson H . CENP-I is essential for centromere function in vertebrate cells. Dev Cell. 2002; 2(4):463-76. DOI: 10.1016/s1534-5807(02)00144-2. View

12.

Talbert P, Masuelli R, Tyagi A, Comai L, Henikoff S . Centromeric localization and adaptive evolution of an Arabidopsis histone H3 variant. Plant Cell. 2002; 14(5):1053-66. PMC: 150606. DOI: 10.1105/tpc.010425. View

13.

Amor D, Bentley K, Ryan J, Perry J, Wong L, Slater H . Human centromere repositioning "in progress". Proc Natl Acad Sci U S A. 2004; 101(17):6542-7. PMC: 404081. DOI: 10.1073/pnas.0308637101. View

14.

Henikoff S, Ahmad K, Malik H . The centromere paradox: stable inheritance with rapidly evolving DNA. Science. 2001; 293(5532):1098-102. DOI: 10.1126/science.1062939. View

15.

Nagaki K, Talbert P, Zhong C, Dawe R, Henikoff S, Jiang J . Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres. Genetics. 2003; 163(3):1221-5. PMC: 1462492. DOI: 10.1093/genetics/163.3.1221. View

16.

Takahashi K, Chen E, Yanagida M . Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast. Science. 2000; 288(5474):2215-9. DOI: 10.1126/science.288.5474.2215. View

17.

Nagaki K, Cheng Z, Ouyang S, Talbert P, Kim M, Jones K . Sequencing of a rice centromere uncovers active genes. Nat Genet. 2004; 36(2):138-45. DOI: 10.1038/ng1289. View

18.

Zhong C, Marshall J, Topp C, Mroczek R, Kato A, Nagaki K . Centromeric retroelements and satellites interact with maize kinetochore protein CENH3. Plant Cell. 2002; 14(11):2825-36. PMC: 152730. DOI: 10.1105/tpc.006106. View

19.

Sugata N, Li S, Earnshaw W, Yen T, Yoda K, Masumoto H . Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere--kinetochore complexes. Hum Mol Genet. 2000; 9(19):2919-26. DOI: 10.1093/hmg/9.19.2919. View

20.

Liu S, Hittle J, Jablonski S, Campbell M, Yoda K, Yen T . Human CENP-I specifies localization of CENP-F, MAD1 and MAD2 to kinetochores and is essential for mitosis. Nat Cell Biol. 2003; 5(4):341-5. DOI: 10.1038/ncb953. View