A Maize Homolog of Mammalian CENPC is a Constitutive Component of the Inner Kinetochore

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 1999 Jul 13

PMID 10402425

Citations 61

Authors

R K Dawe

L M Reed

H G Yu

M G Muszynski

E N Hiatt

Affiliations

Soon will be listed here.

Abstract

Genes for three maize homologs (CenpcA, CenpcB, and CenpcC) of the conserved kinetochore assembly protein known as centromere protein C (CENPC) have been identified. The C-terminal portion of maize CENPC shares similarity with mammalian CENPC and its yeast homolog Mif2p over a 23-amino acid region known as region I. Immunolocalization experiments combined with three-dimensional light microscopy demonstrated that CENPC is a component of the kinetochore throughout interphase, mitosis, and meiosis. It is shown that sister kinetochore separation occurs in two discrete phases during meiosis. A partial separation of sister kinetochores occurs in prometaphase I, and a complete separation occurs in prometaphase II. CENPC is absent on structures known as neocentromeres that, in maize, demonstrate poleward movement but lack other important features of centromeres/kinetochores. CENPC and a previously identified centromeric DNA sequence interact closely but do not strictly colocalize on meiotic chromosomes. These and other data indicate that CENPC occupies an inner domain of the maize kinetochore.

Citing Articles

Mechanisms, Machinery, and Dynamics of Chromosome Segregation in .

Duffy M, Ngaw M, Polsky S, Marzec A, Zhang S, Dzierzgowski O Genes (Basel). 2025; 15(12.

PMID: 39766873 PMC: 11675298. DOI: 10.3390/genes15121606.

The structure, function, and evolution of plant centromeres.

Naish M, Henderson I Genome Res. 2024; 34(2):161-178.

PMID: 38485193 PMC: 10984392. DOI: 10.1101/gr.278409.123.

KNL1 and NDC80 represent new universal markers for the detection of functional centromeres in plants.

Oliveira L, Neumann P, Mata-Sucre Y, Kuo Y, Marques A, Schubert V Chromosome Res. 2024; 32(1):3.

PMID: 38403686 DOI: 10.1007/s10577-024-09747-x.

Exploring Plant Meiosis: Insights from the Kinetochore Perspective.

Zhou K, Zhang C, Niu F, Bai H, Wu D, Deng J Curr Issues Mol Biol. 2023; 45(10):7974-7995.

PMID: 37886947 PMC: 10605258. DOI: 10.3390/cimb45100504.

Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution.

Sridhar S, Fukagawa T Front Cell Dev Biol. 2022; 10:862637.

PMID: 35800888 PMC: 9252888. DOI: 10.3389/fcell.2022.862637.

References

Allshire R . Centromeres, checkpoints and chromatid cohesion. Curr Opin Genet Dev. 1997; 7(2):264-73. DOI: 10.1016/s0959-437x(97)80137-2. View

Asai D, Brokaw C, Thompson W, Wilson L . Two different monoclonal antibodies to alpha-tubulin inhibit the bending of reactivated sea urchin spermatozoa. Cell Motil. 1982; 2(6):599-614. DOI: 10.1002/cm.970020608. View

Saitoh H, Tomkiel J, Cooke C, Ratrie 3rd H, Maurer M, Rothfield N . CENP-C, an autoantigen in scleroderma, is a component of the human inner kinetochore plate. Cell. 1992; 70(1):115-25. DOI: 10.1016/0092-8674(92)90538-n. View

Moens P, Spyropoulos B . Immunocytology of chiasmata and chromosomal disjunction at mouse meiosis. Chromosoma. 1995; 104(3):175-82. DOI: 10.1007/BF00352182. View

Rieder C . The formation, structure, and composition of the mammalian kinetochore and kinetochore fiber. Int Rev Cytol. 1982; 79:1-58. DOI: 10.1016/s0074-7696(08)61672-1. View

Tomkiel J, Cooke C, Saitoh H, Bernat R, Earnshaw W . CENP-C is required for maintaining proper kinetochore size and for a timely transition to anaphase. J Cell Biol. 1994; 125(3):531-45. PMC: 2119987. DOI: 10.1083/jcb.125.3.531. View

Knehr M, Poppe M, Schroeter D, Eickelbaum W, Finze E, Kiesewetter U . Cellular expression of human centromere protein C demonstrates a cyclic behavior with highest abundance in the G1 phase. Proc Natl Acad Sci U S A. 1996; 93(19):10234-9. PMC: 38367. DOI: 10.1073/pnas.93.19.10234. View

Dawe R, Cande W . Induction of centromeric activity in maize by suppressor of meiotic drive 1. Proc Natl Acad Sci U S A. 1996; 93(16):8512-7. PMC: 38703. DOI: 10.1073/pnas.93.16.8512. View

Yang C, Tomkiel J, Saitoh H, Johnson D, Earnshaw W . Identification of overlapping DNA-binding and centromere-targeting domains in the human kinetochore protein CENP-C. Mol Cell Biol. 1996; 16(7):3576-86. PMC: 231353. DOI: 10.1128/MCB.16.7.3576. View

10.

Campbell M, Gorbsky G . Microinjection of mitotic cells with the 3F3/2 anti-phosphoepitope antibody delays the onset of anaphase. J Cell Biol. 1995; 129(5):1195-204. PMC: 2120457. DOI: 10.1083/jcb.129.5.1195. View

11.

Brown M . Sequence similarities between the yeast chromosome segregation protein Mif2 and the mammalian centromere protein CENP-C. Gene. 1995; 160(1):111-6. DOI: 10.1016/0378-1119(95)00163-z. View

12.

Fukagawa T, Brown W . Efficient conditional mutation of the vertebrate CENP-C gene. Hum Mol Genet. 1998; 6(13):2301-8. DOI: 10.1093/hmg/6.13.2301. View

13.

NICKLAS R . How cells get the right chromosomes. Science. 1997; 275(5300):632-7. DOI: 10.1126/science.275.5300.632. View

14.

Harrington J, Van Bokkelen G, Mays R, Gustashaw K, Willard H . Formation of de novo centromeres and construction of first-generation human artificial microchromosomes. Nat Genet. 1997; 15(4):345-55. DOI: 10.1038/ng0497-345. View

15.

Choo K . Centromere DNA dynamics: latent centromeres and neocentromere formation. Am J Hum Genet. 1997; 61(6):1225-33. PMC: 1716064. DOI: 10.1086/301657. View

16.

Alfenito M, Birchler J . Molecular characterization of a maize B chromosome centric sequence. Genetics. 1993; 135(2):589-97. PMC: 1205658. DOI: 10.1093/genetics/135.2.589. View

17.

Pluta A, Mackay A, Ainsztein A, Goldberg I, Earnshaw W . The centromere: hub of chromosomal activities. Science. 1995; 270(5242):1591-4. DOI: 10.1126/science.270.5242.1591. View

18.

Sugimoto K, Yata H, Muro Y, Himeno M . Human centromere protein C (CENP-C) is a DNA-binding protein which possesses a novel DNA-binding motif. J Biochem. 1994; 116(4):877-81. DOI: 10.1093/oxfordjournals.jbchem.a124610. View

19.

Dawe R, Sedat J, Agard D, Cande W . Meiotic chromosome pairing in maize is associated with a novel chromatin organization. Cell. 1994; 76(5):901-12. DOI: 10.1016/0092-8674(94)90364-6. View

20.

Richards E, Dawe R . Plant centromeres: structure and control. Curr Opin Plant Biol. 1999; 1(2):130-5. DOI: 10.1016/s1369-5266(98)80014-9. View