Genetic and Molecular Characterization of the I Locus of Phaseolus Vulgaris

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2005 Dec 3

PMID 16322513

Citations 39

Authors

C Eduardo Vallejos

Gustavo Astua-Monge

Valerie Jones

Tammy R Plyler

Ney S Sakiyama

Sally A Mackenzie

Affiliations

Soon will be listed here.

Abstract

The I locus of the common bean, Phaseolus vulgaris, controls the development of four different phenotypes in response to inoculation with Bean common mosaic virus, Bean common mosaic necrosis virus, several other related potyviruses, and one comovirus. We have generated a high-resolution linkage map around this locus and have aligned it with a physical map constructed with BAC clones. These clones were obtained from a library of the cultivar "Sprite," which carries the dominant allele at the I locus. We have identified a large cluster of TIR-NBS-LRR sequences associated within this locus, which extends over a distance >425 kb. Bean cultivars from the Andean or Mesoamerican gene pool that contain the dominant allele share the same haplotypes as revealed by gel blot hybridizations with a TIR probe. In contrast, beans with a recessive allele display simpler and variable haplotypes. A survey of wild accessions from Argentina to Mexico showed that this multigene family has expanded significantly during evolution and domestication. RNA gel blot analysis indicated that the TIR family of genes plays a role in the response to inoculations with BCMV or BCMNV.

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