Bipartite Signal for Read-through Suppression in Murine Leukemia Virus MRNA: an Eight-nucleotide Purine-rich Sequence Immediately Downstream of the Gag Termination Codon Followed by an RNA Pseudoknot

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1992 Aug 1

PMID 1629968

Citations 58

Authors

Y X Feng

H Yuan

A Rein

J G LEVIN

Affiliations

Soon will be listed here.

Abstract

The pol gene of murine leukemia virus and other mammalian type C retroviruses is expressed by read-through suppression of an in-frame UAG codon which separates the gag and pol coding regions. In this study, we have analyzed the sequence requirements for read-through suppression by placing different portions of wild-type and mutant viral sequences from the gag-pol junction between reporter genes and testing transcripts of these constructs for suppression in reticulocyte lysates. We find that the read-through signal is contained within the first 57 nucleotides on the 3' side of the UAG codon. Our results indicate that the identities of six conserved bases in the eight-nucleotide, purine-rich sequence immediately downstream of the UAG codon are critical for suppression, as is the existence of a pseudoknot structure spanning the next 49 nucleotides. Thus, read-through suppression depends on a complex, bipartite signal in the mRNA.

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