Evidence That TRPC1 Contributes to Calcium-induced Differentiation of Human Keratinocytes

Overview

Journal Pflugers Arch

Specialty Physiology

Date 2005 Nov 12

PMID 16283206

Citations 30

Authors

Shiwei Cai

Sahba Fatherazi

Richard B Presland

Carol M Belton

Frank A Roberts

Paul C Goodwin

Mark M Schubert

Kenneth T Izutsu

Affiliations

Soon will be listed here.

Abstract

External calcium ion concentration is a major regulator of epidermal keratinocyte differentiation in vitro and probably also in vivo. Regulation of calcium-induced differentiation changes is proposed to occur via an external calcium-sensing, signaling pathway that utilizes increases in intracellular calcium ion concentration to activate differentiation-related gene expression. Calcium ion release from intracellular stores and calcium ion influx via store-operated calcium-permeable channels are key elements in this proposed signaling pathway; however, the channels involved have not yet been identified. The present report shows that human gingival keratinocytes (HGKs) also undergo calcium-induced differentiation in vitro as indicated by involucrin expression and morphological changes. Moreover, TRPC1, which functions as a store-operated calcium channel in a number of cell types, including epidermal keratinocytes, is expressed in both proliferating and differentiating HGKs. Transfection of HGKs with TRPC1 siRNA disrupted expression of TRPC1 mRNA and protein compared with transfection with scrambled TRPC1 siRNA. Cells with disrupted TRPC1 expression showed decreased calcium-induced differentiation as measured by involucrin expression or morphological changes, as well as decreased thapsigargin-induced calcium ion influx, and a decreased rate of store calcium release. These results indicate that TRPC1 is involved in calcium-induced differentiation of HGKs likely by supporting a store-operated calcium ion influx.

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References

Sweeney M, Yu Y, Platoshyn O, Zhang S, McDaniel S, Yuan J . Inhibition of endogenous TRP1 decreases capacitative Ca2+ entry and attenuates pulmonary artery smooth muscle cell proliferation. Am J Physiol Lung Cell Mol Physiol. 2002; 283(1):L144-55. DOI: 10.1152/ajplung.00412.2001. View

Komuves L, Oda Y, Tu C, Chang W, Ho-Pao C, Mauro T . Epidermal expression of the full-length extracellular calcium-sensing receptor is required for normal keratinocyte differentiation. J Cell Physiol. 2002; 192(1):45-54. DOI: 10.1002/jcp.10107. View

Mori Y, Wakamori M, Miyakawa T, Hermosura M, Hara Y, Nishida M . Transient receptor potential 1 regulates capacitative Ca(2+) entry and Ca(2+) release from endoplasmic reticulum in B lymphocytes. J Exp Med. 2002; 195(6):673-81. PMC: 2193746. DOI: 10.1084/jem.20011758. View

Mauro T, Bench G, Feingold K, Elias P, Cullander C . Acute barrier perturbation abolishes the Ca2+ and K+ gradients in murine epidermis: quantitative measurement using PIXE. J Invest Dermatol. 1998; 111(6):1198-201. DOI: 10.1046/j.1523-1747.1998.00421.x. View

Wu X, Zagranichnaya T, Gurda G, Eves E, Villereal M . A TRPC1/TRPC3-mediated increase in store-operated calcium entry is required for differentiation of H19-7 hippocampal neuronal cells. J Biol Chem. 2004; 279(42):43392-402. DOI: 10.1074/jbc.M408959200. View

Fatherazi S, Belton C, Cai S, Zarif S, Goodwin P, Lamont R . Calcium receptor message, expression and function decrease in differentiating keratinocytes. Pflugers Arch. 2004; 448(1):93-104. DOI: 10.1007/s00424-003-1223-8. View

Sharpe G, Gillespie J, Greenwell J . An increase in intracellular free calcium is an early event during differentiation of cultured human keratinocytes. FEBS Lett. 1989; 254(1-2):25-8. DOI: 10.1016/0014-5793(89)81002-6. View

Liu X, Singh B, Ambudkar I . TRPC1 is required for functional store-operated Ca2+ channels. Role of acidic amino acid residues in the S5-S6 region. J Biol Chem. 2003; 278(13):11337-43. DOI: 10.1074/jbc.M213271200. View

Gibson D, Ratnam A, Bikle D . Evidence for separate control mechanisms at the message, protein, and enzyme activation levels for transglutaminase during calcium-induced differentiation of normal and transformed human keratinocytes. J Invest Dermatol. 1996; 106(1):154-61. DOI: 10.1111/1523-1747.ep12329856. View

10.

Xu X, Li H, Guggino W, Montell C . Coassembly of TRP and TRPL produces a distinct store-operated conductance. Cell. 1997; 89(7):1155-64. DOI: 10.1016/s0092-8674(00)80302-5. View

11.

La Celle P, Polakowska R . Human homeobox HOXA7 regulates keratinocyte transglutaminase type 1 and inhibits differentiation. J Biol Chem. 2001; 276(35):32844-53. DOI: 10.1074/jbc.M104598200. View

12.

Belton C, Izutsu K, Goodwin P, Park Y, Lamont R . Fluorescence image analysis of the association between Porphyromonas gingivalis and gingival epithelial cells. Cell Microbiol. 2001; 1(3):215-23. DOI: 10.1046/j.1462-5822.1999.00022.x. View

13.

Tu C, Chang W, Bikle D . Phospholipase cgamma1 is required for activation of store-operated channels in human keratinocytes. J Invest Dermatol. 2005; 124(1):187-97. DOI: 10.1111/j.0022-202X.2004.23544.x. View

14.

Brownlow S, Harper A, Harper M, Sage S . A role for hTRPC1 and lipid raft domains in store-mediated calcium entry in human platelets. Cell Calcium. 2004; 35(2):107-13. DOI: 10.1016/j.ceca.2003.08.002. View

15.

Freyssinet J, Martinez M . Loss of plasma membrane phospholipid asymmetry requires raft integrity. Role of transient receptor potential channels and ERK pathway. J Biol Chem. 2002; 277(22):19876-81. DOI: 10.1074/jbc.M200324200. View

16.

Beech D, Xu S, McHugh D, Flemming R . TRPC1 store-operated cationic channel subunit. Cell Calcium. 2003; 33(5-6):433-40. DOI: 10.1016/s0143-4160(03)00054-x. View

17.

Rosado J, Sage S . Activation of store-mediated calcium entry by secretion-like coupling between the inositol 1,4,5-trisphosphate receptor type II and human transient receptor potential (hTrp1) channels in human platelets. Biochem J. 2001; 356(Pt 1):191-8. PMC: 1221827. DOI: 10.1042/0264-6021:3560191. View

18.

Sumitomo S, Kumasa S, Iwai Y, Mori M . Involucrin expression in epithelial tumors of oral and pharyngeal mucosa and skin. Oral Surg Oral Med Oral Pathol. 1986; 62(2):155-63. DOI: 10.1016/0030-4220(86)90038-1. View

19.

Wu X, Babnigg G, Zagranichnaya T, Villereal M . The role of endogenous human Trp4 in regulating carbachol-induced calcium oscillations in HEK-293 cells. J Biol Chem. 2002; 277(16):13597-608. DOI: 10.1074/jbc.M110881200. View

20.

Wang W, OConnell B, Dykeman R, Sakai T, Delporte C, Swaim W . Cloning of Trp1beta isoform from rat brain: immunodetection and localization of the endogenous Trp1 protein. Am J Physiol. 1999; 276(4):C969-79. DOI: 10.1152/ajpcell.1999.276.4.C969. View