Retroviral Restriction Factor TRIM5alpha is a Trimer

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2005 Oct 29

PMID 16254380

Citations 85

Authors

Claudia C Mische

Hassan Javanbakht

Byeongwoon Song

Felipe Diaz-Griffero

Matthew Stremlau

Bettina Strack

Zhihai Si

Joseph Sodroski

Affiliations

Soon will be listed here.

Abstract

The retrovirus restriction factor TRIM5alpha targets the viral capsid soon after entry. Here we show that the TRIM5alpha protein oligomerizes into trimers. The TRIM5alpha coiled-coil and B30.2(SPRY) domains make important contributions to the formation and/or stability of the trimers. A functionally defective TRIM5alpha mutant with the RING and B-box 2 domains deleted can form heterotrimers with wild-type TRIM5alpha, accounting for the observed dominant-negative activity of the mutant protein. Trimerization potentially allows TRIM5alpha to interact with threefold pseudosymmetrical structures on retroviral capsids.

Citing Articles

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The pyrin inflammasome and the Yersinia effector interaction.

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A putative SUMO interacting motif in the B30.2/SPRY domain of rhesus macaque TRIM5α important for NF-κB/AP-1 signaling and HIV-1 restriction.

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References

Li S, Hill C, Sundquist W, Finch J . Image reconstructions of helical assemblies of the HIV-1 CA protein. Nature. 2000; 407(6802):409-13. DOI: 10.1038/35030177. View

Himathongkham S, Luciw P . Restriction of HIV-1 (subtype B) replication at the entry step in rhesus macaque cells. Virology. 1996; 219(2):485-8. DOI: 10.1006/viro.1996.0276. View

Sawyer S, Wu L, Emerman M, Malik H . Positive selection of primate TRIM5alpha identifies a critical species-specific retroviral restriction domain. Proc Natl Acad Sci U S A. 2005; 102(8):2832-7. PMC: 549489. DOI: 10.1073/pnas.0409853102. View

Zhai L, Dietrich A, Skurat A, Roach P . Structure-function analysis of GNIP, the glycogenin-interacting protein. Arch Biochem Biophys. 2004; 421(2):236-42. DOI: 10.1016/j.abb.2003.11.017. View

Towers G, Hatziioannou T, Cowan S, Goff S, Luban J, Bieniasz P . Cyclophilin A modulates the sensitivity of HIV-1 to host restriction factors. Nat Med. 2003; 9(9):1138-43. DOI: 10.1038/nm910. View

Perron M, Stremlau M, Song B, Ulm W, Mulligan R, Sodroski J . TRIM5alpha mediates the postentry block to N-tropic murine leukemia viruses in human cells. Proc Natl Acad Sci U S A. 2004; 101(32):11827-32. PMC: 511059. DOI: 10.1073/pnas.0403364101. View

Nisole S, Lynch C, Stoye J, Yap M . A Trim5-cyclophilin A fusion protein found in owl monkey kidney cells can restrict HIV-1. Proc Natl Acad Sci U S A. 2004; 101(36):13324-8. PMC: 516566. DOI: 10.1073/pnas.0404640101. View

Cowan S, Hatziioannou T, Cunningham T, Muesing M, Gottlinger H, Bieniasz P . Cellular inhibitors with Fv1-like activity restrict human and simian immunodeficiency virus tropism. Proc Natl Acad Sci U S A. 2002; 99(18):11914-9. PMC: 129368. DOI: 10.1073/pnas.162299499. View

Vajdos F, Yoo S, Houseweart M, Sundquist W, Hill C . Crystal structure of cyclophilin A complexed with a binding site peptide from the HIV-1 capsid protein. Protein Sci. 1998; 6(11):2297-307. PMC: 2143596. DOI: 10.1002/pro.5560061103. View

10.

Owens C, Yang P, Gottlinger H, Sodroski J . Human and simian immunodeficiency virus capsid proteins are major viral determinants of early, postentry replication blocks in simian cells. J Virol. 2002; 77(1):726-31. PMC: 140632. DOI: 10.1128/jvi.77.1.726-731.2003. View

11.

Kootstra N, Munk C, Tonnu N, Landau N, Verma I . Abrogation of postentry restriction of HIV-1-based lentiviral vector transduction in simian cells. Proc Natl Acad Sci U S A. 2003; 100(3):1298-303. PMC: 298767. DOI: 10.1073/pnas.0337541100. View

12.

Sokolskaja E, Sayah D, Luban J . Target cell cyclophilin A modulates human immunodeficiency virus type 1 infectivity. J Virol. 2004; 78(23):12800-8. PMC: 524981. DOI: 10.1128/JVI.78.23.12800-12808.2004. View

13.

Peng H, Feldman I, Rauscher 3rd F . Hetero-oligomerization among the TIF family of RBCC/TRIM domain-containing nuclear cofactors: a potential mechanism for regulating the switch between coactivation and corepression. J Mol Biol. 2002; 320(3):629-44. DOI: 10.1016/S0022-2836(02)00477-1. View

14.

Peng H, Begg G, Schultz D, Friedman J, Jensen D, Speicher D . Reconstitution of the KRAB-KAP-1 repressor complex: a model system for defining the molecular anatomy of RING-B box-coiled-coil domain-mediated protein-protein interactions. J Mol Biol. 2000; 295(5):1139-62. DOI: 10.1006/jmbi.1999.3402. View

15.

Gamble T, Vajdos F, Yoo S, Worthylake D, Houseweart M, Sundquist W . Crystal structure of human cyclophilin A bound to the amino-terminal domain of HIV-1 capsid. Cell. 1996; 87(7):1285-94. DOI: 10.1016/s0092-8674(00)81823-1. View

16.

Harbury P, Tidor B, Kim P . Repacking protein cores with backbone freedom: structure prediction for coiled coils. Proc Natl Acad Sci U S A. 1995; 92(18):8408-12. PMC: 41166. DOI: 10.1073/pnas.92.18.8408. View

17.

Lupas A, Van Dyke M, Stock J . Predicting coiled coils from protein sequences. Science. 1991; 252(5009):1162-4. DOI: 10.1126/science.252.5009.1162. View

18.

Sayah D, Sokolskaja E, Berthoux L, Luban J . Cyclophilin A retrotransposition into TRIM5 explains owl monkey resistance to HIV-1. Nature. 2004; 430(6999):569-73. DOI: 10.1038/nature02777. View

19.

Harbury P, Zhang T, Kim P, Alber T . A switch between two-, three-, and four-stranded coiled coils in GCN4 leucine zipper mutants. Science. 1993; 262(5138):1401-7. DOI: 10.1126/science.8248779. View

20.

Besnier C, Takeuchi Y, Towers G . Restriction of lentivirus in monkeys. Proc Natl Acad Sci U S A. 2002; 99(18):11920-5. PMC: 129369. DOI: 10.1073/pnas.172384599. View