Dicer-1 and R3D1-L Catalyze MicroRNA Maturation in Drosophila

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2005 Jun 30

PMID 15985611

Citations 151

Authors

Feng Jiang

Xuecheng Ye

Xiang Liu

Lauren Fincher

Dennis McKearin

Qinghua Liu

Affiliations

Soon will be listed here.

Abstract

In Drosophila melanogaster, Dicer-2/R2D2 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively. Here we identify a novel dsRNA-binding protein, which we named R3D1-L, that forms a stable complex with Dicer-1 in vitro and in vivo. While depletion of R3D1-L by RNAi causes accumulation of precursor miRNA (pre-miRNA) in S2 cells, recombinant R3D1-L enhances miRNA production by Dicer-1 in vitro. Furthermore, R3D1 deficiency causes miRNA-generating defect and severe sterility in male and female flies. Therefore, R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development in Drosophila.

Citing Articles

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