Effects of Butyrate Homologues on Metallothionein Induction in Rat Primary Hepatocyte Cultures

Overview

Journal In Vitro Cell Dev Biol

Specialties Anatomy & Histology
Cell Biology

Date 1992 May 11

PMID 1597404

Citations 2

Authors

J Liu

J M McKim Jr

Y P Liu

C D Klaassen

Affiliations

Soon will be listed here.

Abstract

Sodium butyrate (NaB), a 4-carbon fatty acid, has been reported to activate the metallothionein (MT) gene in certain carcinoma cell lines. Because the effects of NaB are dependent on the cell type investigated, this study was conducted to determine if NaB and its homologues induce MT in rat primary hepatocyte cultures. Hepatocytes were grown on monolayer for 12 h and subsequently treated with formate, acetate, propionate (NaP), NaB, and valeric acid for 10 to 58 h. To examine their interaction with known MT inducers, cadmium (Cd), zinc (Zn), or dexamethasone (Dex) were added to some cultures. MT protein in the cells was quantitated by the Cd-hemoglobin assay; MT-1 mRNA was analyzed by Northern blot hybridizations with oligonucleotide probes, and quantitated by slot-blot analysis. Among the 1 to 5 carbon carboxylic acids, only NaP (3 carbon) and NaB (4 carbon) induced MT. NaP and NaB alone produced a moderate increase in MT two- to fourfold over control), but when combined with Cd or Dex, an additive increase was observed. However, when combined with Zn, a synergistic increase was detected. NaB and Zn synergistically increased MT protein, but produced only an additive increase in MT mRNA, suggesting the involvement of some posttranscriptional event(s) in the NaB-Zn induction of MT. In conclusion, NaP and NaB induced MT in normal cultured rat hepatocytes, producing an additive increase in MT protein with Cd and Dex, and a synergistic increase in MT protein with Zn.

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References

Andrews G . Regulation of metallothionein gene expression. Prog Food Nutr Sci. 1990; 14(2-3):193-258. View

Andrews G, Adamson E . Butyrate selectively activates the metallothionein gene in teratocarcinoma cells and induces hypersensitivity to metal induction. Nucleic Acids Res. 1987; 15(13):5461-75. PMC: 305972. DOI: 10.1093/nar/15.13.5461. View

Bracken W, Klaassen C . Induction of metallothionein in rat primary hepatocyte cultures: evidence for direct and indirect induction. J Toxicol Environ Health. 1987; 22(2):163-74. DOI: 10.1080/15287398709531060. View

Berry M, Friend D . High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol. 1969; 43(3):506-20. PMC: 2107801. DOI: 10.1083/jcb.43.3.506. View

Engelmann G, Staecker J, Richardson A . Effect of sodium butyrate on primary cultures of adult rat hepatocytes. In Vitro Cell Dev Biol. 1987; 23(2):86-92. DOI: 10.1007/BF02623587. View

Nakamura T, Ichihara A . Control of growth and expression of differentiated functions of mature hepatocytes in primary culture. Cell Struct Funct. 1985; 10(1):1-16. DOI: 10.1247/csf.10.1. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Prasad K . Butyric acid: a small fatty acid with diverse biological functions. Life Sci. 1980; 27(15):1351-8. DOI: 10.1016/0024-3205(80)90397-5. View

Eaton D, Toal B . Evaluation of the Cd/hemoglobin affinity assay for the rapid determination of metallothionein in biological tissues. Toxicol Appl Pharmacol. 1982; 66(1):134-42. DOI: 10.1016/0041-008x(82)90068-0. View

10.

Dunn M, Blalock T, Cousins R . Metallothionein. Proc Soc Exp Biol Med. 1987; 185(2):107-19. DOI: 10.3181/00379727-185-42525a. View

11.

Kreamer B, Staecker J, Sawada N, Sattler G, Hsia M, Pitot H . Use of a low-speed, iso-density percoll centrifugation method to increase the viability of isolated rat hepatocyte preparations. In Vitro Cell Dev Biol. 1986; 22(4):201-11. DOI: 10.1007/BF02623304. View

12.

Prasad K, Sinha P . Effect of sodium butyrate on mammalian cells in culture: a review. In Vitro. 1976; 12(2):125-32. DOI: 10.1007/BF02796360. View

13.

Collins M, Hunsaker W . Improved hybridization assays employing tailed oligonucleotide probes: a direct comparison with 5'-end-labeled oligonucleotide probes and nick-translated plasmid probes. Anal Biochem. 1985; 151(2):211-24. DOI: 10.1016/0003-2697(85)90168-x. View

14.

Andersen R, Birren B, Taplitz S, Herschman H . Rat metallothionein-1 structural gene and three pseudogenes, one of which contains 5'-regulatory sequences. Mol Cell Biol. 1986; 6(1):302-14. PMC: 367511. DOI: 10.1128/mcb.6.1.302-314.1986. View

15.

Waalkes M, Wilson M . Enhancement of cadmium-induced metallothionein synthesis in cultured TRL 1215 cells by butyric acid pretreatment. Toxicol Lett. 1986; 32(3):289-94. DOI: 10.1016/0378-4274(86)90121-9. View

16.

Staecker J, Sattler C, Pitot H . Sodium butyrate preserves aspects of the differentiated phenotype of normal adult rat hepatocytes in culture. J Cell Physiol. 1988; 135(3):367-76. DOI: 10.1002/jcp.1041350303. View

17.

Birren B, Herschman H . Regulation of the rat metallothionein-I gene by sodium butyrate. Nucleic Acids Res. 1986; 14(2):853-67. PMC: 339469. DOI: 10.1093/nar/14.2.853. View

18.

BISSELL D, Guzelian P . Phenotypic stability of adult rat hepatocytes in primary monolayer culture. Ann N Y Acad Sci. 1980; 349:85-98. DOI: 10.1111/j.1749-6632.1980.tb29518.x. View

19.

Cousins R . Absorption, transport, and hepatic metabolism of copper and zinc: special reference to metallothionein and ceruloplasmin. Physiol Rev. 1985; 65(2):238-309. DOI: 10.1152/physrev.1985.65.2.238. View

20.

Thorne A, Kmiciek D, Mitchelson K, Sautiere P, Crane-Robinson C . Patterns of histone acetylation. Eur J Biochem. 1990; 193(3):701-13. DOI: 10.1111/j.1432-1033.1990.tb19390.x. View