A Single Polypeptide Catalyzing the Conversion of Phytoene to Zeta-carotene is Transcriptionally Regulated During Tomato Fruit Ripening

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1992 Jun 1

PMID 1594600

Citations 55

Authors

I PECKER

D Chamovitz

H Linden

G Sandmann

J Hirschberg

Affiliations

Soon will be listed here.

Abstract

The cDNA of the gene pds from tomato, encoding the carotenoid biosynthesis enzyme phytoene desaturase, was cloned, and its nucleotide sequence was determined. Cells of Escherichia coli that expressed the tomato pds gene could convert phytoene to zeta-carotene. This result suggests that one polypeptide, the product of the pds gene, can carry out phytoene desaturation in the carotenoid biosynthetic pathway. Transcripts of the pds gene accumulate in orange tomato fruit, indicating transcriptional control of pds expression during fruit ripening. The deduced amino acid sequence of phytoene desaturase indicates that this enzyme in tomato contains 583 amino acids that are highly conserved with respect to the homologous enzymes in cyanobacteria and algae. The deduced amino acid sequences of the phytoene desaturases from other microorganisms (purple bacteria and fungi) appear to be evolutionarily unrelated to those from green photosynthetic organisms.

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References

Kuriyan J, Krishna T, Wong L, Guenther B, Pahler A, Williams Jr C . Convergent evolution of similar function in two structurally divergent enzymes. Nature. 1991; 352(6331):172-4. DOI: 10.1038/352172a0. View

Wright C, Alden R, KRAUT J . Structure of subtilisin BPN' at 2.5 angström resolution. Nature. 1969; 221(5177):235-42. DOI: 10.1038/221235a0. View

Misawa N, Nakagawa M, Kobayashi K, Yamano S, Izawa Y, Nakamura K . Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J Bacteriol. 1990; 172(12):6704-12. PMC: 210783. DOI: 10.1128/jb.172.12.6704-6712.1990. View

Krinsky N . Carotenoids and cancer in animal models. J Nutr. 1989; 119(1):123-6. DOI: 10.1093/jn/119.1.123. View

Armstrong G, Alberti M, Leach F, Hearst J . Nucleotide sequence, organization, and nature of the protein products of the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus. Mol Gen Genet. 1989; 216(2-3):254-68. DOI: 10.1007/BF00334364. View

Bartley G, Scolnik P . Carotenoid biosynthesis in photosynthetic bacteria. Genetic characterization of the Rhodobacter capsulatus CrtI protein. J Biol Chem. 1989; 264(22):13109-13. View

Sandmann G, WARD C, Lo W, Nagy J, Boger P . Bleaching herbicide flurtamone interferes with phytoene desaturase. Plant Physiol. 1990; 94(2):476-8. PMC: 1077256. DOI: 10.1104/pp.94.2.476. View

Chamovitz D, PECKER I, Hirschberg J . The molecular basis of resistance to the herbicide norflurazon. Plant Mol Biol. 1991; 16(6):967-74. DOI: 10.1007/BF00016069. View

Bartley G, Viitanen P, PECKER I, Chamovitz D, Hirschberg J, Scolnik P . Molecular cloning and expression in photosynthetic bacteria of a soybean cDNA coding for phytoene desaturase, an enzyme of the carotenoid biosynthesis pathway. Proc Natl Acad Sci U S A. 1991; 88(15):6532-6. PMC: 52120. DOI: 10.1073/pnas.88.15.6532. View

10.

Sandmann G, Kowalczyk S . In vitro carotenogenesis and characterization of the phytoene desaturase reaction in Anacystis. Biochem Biophys Res Commun. 1989; 163(2):916-21. DOI: 10.1016/0006-291x(89)92309-7. View

11.

Krinsky N . Antioxidant functions of carotenoids. Free Radic Biol Med. 1989; 7(6):617-35. DOI: 10.1016/0891-5849(89)90143-3. View

12.

Bendich A . Carotenoids and the immune response. J Nutr. 1989; 119(1):112-5. DOI: 10.1093/jn/119.1.112. View

13.

Chamovitz D, PECKER I, Sandmann G, Boger P, Hirschberg J . Cloning a gene coding for norflurazon resistance in cyanobacteria. Z Naturforsch C J Biosci. 1990; 45(5):482-6. DOI: 10.1515/znc-1990-0531. View

14.

Schmidhauser T, Lauter F, Russo V, Yanofsky C . Cloning, sequence, and photoregulation of al-1, a carotenoid biosynthetic gene of Neurospora crassa. Mol Cell Biol. 1990; 10(10):5064-70. PMC: 361171. DOI: 10.1128/mcb.10.10.5064-5070.1990. View

15.

Scrutton N, Berry A, Perham R . Redesign of the coenzyme specificity of a dehydrogenase by protein engineering. Nature. 1990; 343(6253):38-43. DOI: 10.1038/343038a0. View

16.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

17.

Aviv H, Leder P . Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972; 69(6):1408-12. PMC: 426713. DOI: 10.1073/pnas.69.6.1408. View

18.

Armstrong G, Alberti M, Hearst J . Conserved enzymes mediate the early reactions of carotenoid biosynthesis in nonphotosynthetic and photosynthetic prokaryotes. Proc Natl Acad Sci U S A. 1990; 87(24):9975-9. PMC: 55297. DOI: 10.1073/pnas.87.24.9975. View