Antimutagenic Properties of Green Tea

Overview

Journal Plant Foods Hum Nutr

Specialties Biology
Nutritional Sciences

Date 2005 May 19

PMID 15898356

Citations 6

Authors

R Bunkova

I Marova

M Nemec

Affiliations

Soon will be listed here.

Abstract

In this work biological effects of two common kinds of green tea (Chinese Gunpowder and Japanese Sencha) were analyzed using three independent tests of antimutagenicity: 1) the Ames test with Salmonella typhimurium TA98, 2) cytogenetic analysis of peripheral blood lymphocytes (CAPL), and 3) test with Saccharomyces cerevisiae D7. Tea extracts were allowed to be antimutagenic based on their ability to inhibit the mutagenic effect of standard mutagens. Amounts of (-)catechin and (-)catechin gallate in tea extracts were determined by high performance liquid chromatography on reversed phase (RP-HPLC). Antioxidant capacity was found using total radical-trapping antioxidant parameter (TRAP) method. Extracts from Gunpowder and Sencha exhibited high antimutagenic activity in the Ames test (24.7+/-3.7% and 34.1+/-2, 1% of inhibition without metabolic activation; 74.9+/-1.7% and 62.7+/-4.3% of inhibition with metabolic activation, respectively) as well as in S. cerevisiae D7 test (Gunpowder: 62.7+/-5.7% of Trp convertants inhibition and 52.6+/-5.3% of Ilv revertants inhibition; Sencha: 45.6+/-4.2% of Trp convertants inhibition, 50.0+/-4.8% of Ilv revertants inhibition). In the CAPL method reduced number of abberant cells as well as decreased number of chromosome breaks was observed using both green tea extracts. Antioxidant capacity and antimutagenicity of green tea extracts was higher than activity of tea catechins and flavonoids.

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References

Kuroda Y, Hara Y . Antimutagenic and anticarcinogenic activity of tea polyphenols. Mutat Res. 1999; 436(1):69-97. DOI: 10.1016/s1383-5742(98)00019-2. View

Beudot C, De Meo M, Dauzonne D, Elias R, Laget M, Guiraud H . Evaluation of the mutagenicity and antimutagenicity of forty-two 3-substituted flavones in the Ames test. Mutat Res. 1998; 417(2-3):141-53. DOI: 10.1016/s1383-5718(98)00103-x. View

Merken H, Beecher G . Measurement of food flavonoids by high-performance liquid chromatography: A review. J Agric Food Chem. 2000; 48(3):577-99. DOI: 10.1021/jf990872o. View

Jodoin J, Demeule M, Beliveau R . Inhibition of the multidrug resistance P-glycoprotein activity by green tea polyphenols. Biochim Biophys Acta. 2002; 1542(1-3):149-59. DOI: 10.1016/s0167-4889(01)00175-6. View

Namiki M . Antioxidants/antimutagens in food. Crit Rev Food Sci Nutr. 1990; 29(4):273-300. DOI: 10.1080/10408399009527528. View

Han C . Screening of anticarcinogenic ingredients in tea polyphenols. Cancer Lett. 1997; 114(1-2):153-8. DOI: 10.1016/s0304-3835(97)04647-8. View

ZIMMERMANN F, VON Borstel R, Von Halle E, Parry J, Siebert D, Zetterberg G . Testing of chemicals for genetic activity with Saccharomyces cerevisiae: a report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutat Res. 1984; 133(3):199-244. DOI: 10.1016/0165-1110(84)90017-4. View

Wall M, Wani M, Hughes T, Taylor H . Plant antimutagenic agents, 1. General bioassay and isolation procedures. J Nat Prod. 1988; 51(5):866-73. DOI: 10.1021/np50059a009. View

Ito Y, Ohnishi S, Fujie K . Chromosome aberrations induced by aflatoxin B1 in rat bone marrow cells in vivo and their suppression by green tea. Mutat Res. 1989; 222(3):253-61. DOI: 10.1016/0165-1218(89)90141-9. View

10.

Roy M, Siddiqi M, Bhattacharya R . Cancer Chemoprevention: Tea Polyphenol Induced Cellular and Molecular Responses. Asian Pac J Cancer Prev. 2003; 2(2):109-116. View

11.

Simic D, Vukovic-Gacic B, Knezevic-Vukcevic J . Detection of natural bioantimutagens and their mechanisms of action with bacterial assay-system. Mutat Res. 1998; 402(1-2):51-7. DOI: 10.1016/s0027-5107(97)00281-9. View

12.

Roy M, Chakrabarty S, Sinha D, Bhattacharya R, Siddiqi M . Anticlastogenic, antigenotoxic and apoptotic activity of epigallocatechin gallate: a green tea polyphenol. Mutat Res. 2003; 523-524:33-41. DOI: 10.1016/s0027-5107(02)00319-6. View

13.

Katiyar S, Mukhtar H . Tea in chemoprevention of cancer. Int J Oncol. 2011; 8(2):221-38. DOI: 10.3892/ijo.8.2.221. View

14.

Slavikova H, Lojek A, Hamar J, Duskova M, Kubala L, Vondracek J . Total antioxidant capacity of serum increased in early but not late period after intestinal ischemia in rats. Free Radic Biol Med. 1998; 25(1):9-18. DOI: 10.1016/s0891-5849(98)00030-6. View

15.

Lambert J, Yang C . Cancer chemopreventive activity and bioavailability of tea and tea polyphenols. Mutat Res. 2003; 523-524:201-8. DOI: 10.1016/s0027-5107(02)00336-6. View

16.

Johnson M, Loo G . Effects of epigallocatechin gallate and quercetin on oxidative damage to cellular DNA. Mutat Res. 2000; 459(3):211-8. DOI: 10.1016/s0921-8777(99)00074-9. View

17.

Ohe T, Marutani K, Nakase S . Catechins are not major components responsible for anti-genotoxic effects of tea extracts against nitroarenes. Mutat Res. 2001; 496(1-2):75-81. DOI: 10.1016/s1383-5718(01)00220-0. View

18.

Dhawan A, Anderson D, de Pascual-Teresa S, Santos-Buelga C, Clifford M, Ioannides C . Evaluation of the antigenotoxic potential of monomeric and dimeric flavanols, and black tea polyphenols against heterocyclic amine-induced DNA damage in human lymphocytes using the Comet assay. Mutat Res. 2002; 515(1-2):39-56. DOI: 10.1016/s1383-5718(01)00347-3. View