Properties of Membranes from Mutant Strains of Escherichia Coli in Which the Beta-subunit of the Adenosine Triphosphatase is Abnormal

Overview

Journal Biochem J

Specialty Biochemistry

Date 1979 Apr 15

PMID 158358

Citations 24

Authors

A E Senior

D R Fayle

J A Downie

F Gibson

G B Cox

Affiliations

Soon will be listed here.

Abstract

Five uncoupled mutant strains of Escherichia coli carrying mutations in the uncD gene have been studied. In each of these mutant strains the beta-subunit of the F1 portion of the membrane-bound adenosine triphosphatase is abnormal. In one of the mutant strains (carrying the uncD12 allele) in F1-ATPase aggregate was formed which was purified and found to have low ATPase activity. ATPase activity was absent in the other four strains and the abnormal beta-subunits were tightly bound to the membranes. However, membranes from these strains exhibited various proton permeabilities as indicated by NADH-dependent atebrin-fluorescence quenching and bound different amounts of normal F1-ATPase. The amounts of reconstitution of energy-linked reactions after the addition of normal F1-ATPase also varied depending on the mutant allele. It is apparent that considerable phenotypic variations can occur between strains carrying mutations in the same unc gene.

Citing Articles

The nucleotide binding affinities of two critical conformations of Escherichia coli ATP synthase.

Li Y, Valdez N, Mnatsakanyan N, Weber J Arch Biochem Biophys. 2021; 707:108899.

PMID: 33991499 PMC: 8278868. DOI: 10.1016/j.abb.2021.108899.

Photosynthetic ATPases: purification, properties, subunit isolation and function.

Merchant S, Selman B Photosynth Res. 2014; 6(1):3-31.

PMID: 24442826 DOI: 10.1007/BF00029044.

Improved crystallization of Escherichia coli ATP synthase catalytic complex (F1) by introducing a phosphomimetic mutation in subunit ε.

Roy A, Hutcheon M, Duncan T, Cingolani G Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012; 68(Pt 10):1229-33.

PMID: 23027754 PMC: 3490465. DOI: 10.1107/S1744309112036718.

Two ATPases.

Senior A J Biol Chem. 2012; 287(36):30049-62.

PMID: 22822068 PMC: 3436262. DOI: 10.1074/jbc.X112.402313.

Sensitivity of tonoplast-bound adenosine-triphosphatase from hevea to inhibitors.

Marin B Plant Physiol. 1983; 73(4):973-7.

PMID: 16663354 PMC: 1066591. DOI: 10.1104/pp.73.4.973.

References

Luria S, BURROUS J . Hybridization between Escherichia coli and Shigella. J Bacteriol. 1957; 74(4):461-76. PMC: 289941. DOI: 10.1128/jb.74.4.461-476.1957. View

Gibson F, Cox G, Downie J, Radik J . Partial diploids of Escherichia coli carrying normal and mutant alleles affecting oxidative phosphorylation. Biochem J. 1977; 162(3):665-70. PMC: 1164651. DOI: 10.1042/bj1620665. View

Gibson F, Cox G, Downie J, Radik J . A mutation affecting a second component of the F0 portion of the magnesium ion-stimulated adenosine triphosphatase of Escherichia coli K12. The uncC424 allele. Biochem J. 1977; 164(1):193-8. PMC: 1164774. DOI: 10.1042/bj1640193. View

Cox G, Downie J, Fayle D, Gibson F, Radik J . Inhibition, by a protease inhibitor, of the solubilization of the F1-portion of the Mg2+-stimulated adenosine triphosphatase of Escherichia coli. J Bacteriol. 1978; 133(1):287-92. PMC: 222006. DOI: 10.1128/jb.133.1.287-292.1978. View

Cox G, Downie J, Gibson F, Radik J . Genetic complementation between two mutant unc alleles (unc A401 and unc D409) affecting the Fl portion of the magnesium ion-stimulated adenosine triphosphatase of Escherichia coli K12. Biochem J. 1978; 170(3):593-8. PMC: 1183936. DOI: 10.1042/bj1700593. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Gibson F, Downie J, Cox G, Radik J . Mu-induced polarity in the unc operon of Escherichia coli. J Bacteriol. 1978; 134(3):728-36. PMC: 222317. DOI: 10.1128/jb.134.3.728-736.1978. View

Fayle D, Downie J, Cox G, Gibson F, Radik J . Characterization of the mutant-unc D-gene product in a strain of Escherichia coli K12. An altered beta-subunit of the magnesium ion-stimulated adenosine triphosphatase. Biochem J. 1978; 172(3):523-31. PMC: 1185727. DOI: 10.1042/bj1720523. View

Senior A, Downie J, Cox G, Gibson F, Langman L, Fayle D . The uncA gene codes for the alpha-subunit of the adenosine triphosphatase of Escherichia coli. Electrophoretic analysis of uncA mutant strains. Biochem J. 1979; 180(1):103-9. PMC: 1161024. DOI: 10.1042/bj1800103. View

10.

Ofarrell P . High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975; 250(10):4007-21. PMC: 2874754. View

11.

Butlin J, Cox G, Gibson F . Oxidative phosphorylation in Escherichia coli K-12: the genetic and biochemical characterisations of a strain carrying a mutation in the uncB gene. Biochim Biophys Acta. 1973; 292(2):366-75. DOI: 10.1016/0005-2728(73)90043-1. View

12.

Kell D, Ferguson S, John P . Measurement by a flow dialysis technique of the steady-state proton-motive force in chromatophores from Rhodospirillum rubrum. Comparison with phosphorylation potential. Biochim Biophys Acta. 1978; 502(1):111-26. DOI: 10.1016/0005-2728(78)90136-6. View

13.

Cox G, Newton N, Gibson F, SNOSWELL A, Hamilton J . The function of ubiquinone in Escherichia coli. Biochem J. 1970; 117(3):551-62. PMC: 1178960. DOI: 10.1042/bj1170551. View

14.

Senior A, Brooks J . Studies on the mitochondrial oligomycin-insensitivt ATPase. I. An improved method of purification and the behavior of the enzyme in solutions of various depolymerizing agents. Arch Biochem Biophys. 1970; 140(1):257-66. DOI: 10.1016/0003-9861(70)90030-5. View

15.

Butlin J, Cox G, Gibson F . Oxidative phosphorylation in Escherichia coli K12. Mutations affecting magnesium ion- or calcium ion-stimulated adenosine triphosphatase. Biochem J. 1971; 124(1):75-81. PMC: 1177115. DOI: 10.1042/bj1240075. View

16.

Cox G, Gibson F, McCann L, Butlin J, Crane F . Reconstitution of the energy-linked transhydrogenase activity in membranes from a mutant strain of Escherichia coli K12 lacking magnesium ion- or calcium ion-stimulated adenosine triphosphatase. Biochem J. 1973; 132(4):689-95. PMC: 1177644. DOI: 10.1042/bj1320689. View

17.

Cox G, Gibson F, McCann L . Reconstitution of oxidative phosphorylation and the adenosine triphosphate-dependent transhydrogenase activity by a combination of membrane fractions from unCA- and uncB- mutant strains of Escherichia coli K12. Biochem J. 1973; 134(4):1015-21. PMC: 1177910. DOI: 10.1042/bj1341015. View

18.

Deamer D, Prince R, Crofts A . The response of fluorescent amines to pH gradients across liposome membranes. Biochim Biophys Acta. 1972; 274(2):323-35. DOI: 10.1016/0005-2736(72)90180-0. View