Sensitivity of Tonoplast-bound Adenosine-triphosphatase from Hevea to Inhibitors

Overview

Journal Plant Physiol

Specialty Physiology

Date 1983 Dec 1

PMID 16663354

Citations 3

Authors

B Marin

Affiliations

Soon will be listed here.

Abstract

The tonoplast-bound H(+)-translocating ATPase from Hevea latex was found to be insensitive to vanadate, diethylstilbestrol, and octylguanidine, which are specific inhibitors of the plasma membrane ATPase. The inhibitors of the mitochondrial ATPase, oligomycin and azide, and also rotenone and antimycin A, were all without effect. In contrast, trimethyltin chloride strongly inhibited the activity of Hevea tonoplast ATPase.Among the different carbodiimides tested, which strongly inhibit the Hevea tonoplast ATPase, N,N'-dicyclohexylcarbodiimide was the most inhibitory. N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline was also an efficient inhibitor.This unique inhibitor sensitivity of the Hevea tonoplast H(+)-translocating ATPase suggests that this enzyme differs in its mode of operation from all other known H(+)-translocating ATPases.

Citing Articles

Sucrose phosphatase associated with vacuole preparations from red beet, sugar beet, and immature sugarcane stem.

Hawker J, Smith G, Phillips H, Wiskich J Plant Physiol. 1987; 84(4):1281-5.

PMID: 16665598 PMC: 1056765. DOI: 10.1104/pp.84.4.1281.

The control by delta mu H+ of the tonoplast-bound H+-translocating adenosine triphosphatase from rubber-tree (Hevea brasiliensis) latex.

Marin B Biochem J. 1985; 229(2):459-67.

PMID: 2994636 PMC: 1145078. DOI: 10.1042/bj2290459.

Chloride-ion stimulation of the tonoplast H+-translocating ATPase from Hevea brasiliensis (rubber tree) latex. A dual mechanism.

Marin B, Gidrol X Biochem J. 1985; 226(1):85-94.

PMID: 2579642 PMC: 1144680. DOI: 10.1042/bj2260085.

References

Maloney P . Energy coupling to ATP synthesis by the proton-translocating ATPase. J Membr Biol. 1982; 67(1):1-12. DOI: 10.1007/BF01868643. View

TAUSSKY H, SHORR E . A microcolorimetric method for the determination of inorganic phosphorus. J Biol Chem. 1953; 202(2):675-85. View

Goffeau A, SLAYMAN C . The proton-translocating ATPase of the fungal plasma membrane. Biochim Biophys Acta. 1981; 639(3-4):197-223. DOI: 10.1016/0304-4173(81)90010-0. View

Lin W, Wagner G, Siegelman H, Hind G . Membrane-bound ATPase of intact vacuoles and tonoplasts isolated from mature plant tissue. Biochim Biophys Acta. 1977; 465(1):110-7. DOI: 10.1016/0005-2736(77)90359-5. View

Bowman B, Mainzer S, Allen K, SLAYMAN C . Effects of inhibitors on the plasma membrane and mitochondrial adenosine triphosphatases of Neurospora crassa. Biochim Biophys Acta. 1978; 512(1):13-28. DOI: 10.1016/0005-2736(78)90214-6. View

Senior A, Fayle D, Downie J, Gibson F, Cox G . Properties of membranes from mutant strains of Escherichia coli in which the beta-subunit of the adenosine triphosphatase is abnormal. Biochem J. 1979; 180(1):111-8. PMC: 1161025. DOI: 10.1042/bj1800111. View

Grubmeyer C, Spencer M . ATPase Activity of Pea Cotyledon Submitochondrial Particles: ACTIVATION, SUBSTRATE SPECIFICITY, AND ANION EFFECTS. Plant Physiol. 1980; 65(2):281-5. PMC: 440311. DOI: 10.1104/pp.65.2.281. View

Okorokov L, Kulakovskaya T, Kulaev I . Solubilization and partial purification of vacuolar ATPase of yeast Saccharomyces carlsbergensis. FEBS Lett. 1982; 145(1):160-2. DOI: 10.1016/0014-5793(82)81227-1. View

Johnson R, Beers M, Scarpa A . H+ ATPase of chromaffin granules. Kinetics, regulation, and stoichiometry. J Biol Chem. 1982; 257(18):10701-7. View

10.

Cretin H . The proton gradient across the vacuo-lysosomal membrane of lutoids from the latex of Hevea brasiliensis. I. Further evidence for a proton-translocating ATPase on the vacuo-lysosomal membrane of intact lutoids. J Membr Biol. 1982; 65(3):175-84. DOI: 10.1007/BF01869961. View

11.

Balke N, Hodges T . Inhibition of adenosine triphosphatase activity of the plasma membrane fraction of oat roots by diethylstilbestrol. Plant Physiol. 1979; 63(1):48-52. PMC: 542763. DOI: 10.1104/pp.63.1.48. View

12.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

13.

Marin B, Komor E . The protonmotive potential difference across the vacuo-lysosomal membrane of Hevea brasiliensis (rubber tree) and its modification by a membrane-bound adenosine triphosphatase. Biochem J. 1981; 198(2):365-72. PMC: 1163257. DOI: 10.1042/bj1980365. View

14.

Cantley Jr L, Josephson L, Warner R, Yanagisawa M, Lechene C, Guidotti G . Vanadate is a potent (Na,K)-ATPase inhibitor found in ATP derived from muscle. J Biol Chem. 1977; 252(21):7421-3. View