Role of Beta2-integrins for Homing and Neovascularization Capacity of Endothelial Progenitor Cells

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 2004 Dec 30

PMID 15623573

Citations 103

Authors

Emmanouil Chavakis

Alexandra Aicher

Christopher Heeschen

Ken-Ichiro Sasaki

Ralf Kaiser

Naual El Makhfi

Carmen Urbich

Thorsten Peters

Karin Scharffetter-Kochanek

Andreas M Zeiher

Triantafyllos Chavakis

Stefanie Dimmeler

Affiliations

Soon will be listed here.

Abstract

The mechanisms of homing of endothelial progenitor cells (EPCs) to sites of ischemia are unclear. Here, we demonstrate that ex vivo-expanded EPCs as well as murine hematopoietic Sca-1+/Lin- progenitor cells express beta2-integrins, which mediate the adhesion of EPCs to endothelial cell monolayers and their chemokine-induced transendothelial migration in vitro. In a murine model of hind limb ischemia, Sca-1+/Lin- hematopoietic progenitor cells from beta2-integrin-deficient mice are less capable of homing to sites of ischemia and of improving neovascularization. Preactivation of the beta2-integrins expressed on EPCs by activating antibodies augments the EPC-induced neovascularization in vivo. These results provide evidence for a novel function of beta2-integrins in postnatal vasculogenesis.

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