HIV-1 Nef Suppression by Virally Encoded MicroRNA

Overview

Journal Retrovirology

Publisher Biomed Central

Specialty Microbiology

Date 2004 Dec 17

PMID 15601474

Citations 131

Authors

Shinya Omoto

Masafumi Ito

Yutaka Tsutsumi

Yuko Ichikawa

Harumi Okuyama

Ebiamadon Andi Brisibe

Nitin K Saksena

Yoichi R Fujii

Affiliations

Soon will be listed here.

Abstract

Background: MicroRNAs (miRNAs) are 21 to approximately 25-nucleotides (nt) long and interact with mRNAs to trigger either translational repression or RNA cleavage through RNA interference (RNAi), depending on the degree of complementarity with the target mRNAs. Our recent study has shown that HIV-1 nef dsRNA from AIDS patients who are long-term non-progressors (LTNPs) inhibited the transcription of HIV-1.

Results: Here, we show the possibility that nef-derived miRNAs are produced in HIV-1 persistently infected cells. Furthermore, nef short hairpin RNA (shRNA) that corresponded to a predicted nef miRNA (approximately 25 nt, miR-N367) can block HIV-1 Nef expression in vitro and the suppression by shRNA/miR-N367 would be related with low viremia in an LTNP (15-2-2). In the 15-2-2 model mice, the weight loss, which may be rendered by nef was also inhibited by shRNA/miR-N367 corresponding to suppression of nef expression in vivo.

Conclusions: These data suggest that nef/U3 miRNAs produced in HIV-1-infected cells may suppress both Nef function and HIV-1 virulence through the RNAi pathway.

Citing Articles

MicroRNAs in HIV infection: dual regulators of viral replication and host immunity.

Mansour R, El-Sayyad G, Rizk N, Mageed S, Basiouny M, El-Sayed S Naunyn Schmiedebergs Arch Pharmacol. 2025; .

PMID: 40029387 DOI: 10.1007/s00210-025-03893-7.

A comprehensive overview on the crosstalk between microRNAs and viral pathogenesis and infection.

Bahojb Mahdavi S, Jebelli A, Aghbash P, Baradaran B, Amini M, Oroojalian F Med Res Rev. 2024; 45(2):349-425.

PMID: 39185567 PMC: 11796338. DOI: 10.1002/med.22073.

The Role of MicroRNAs in HIV Infection.

Morando N, Rosenzvit M, Pando M, Allmer J Genes (Basel). 2024; 15(5).

PMID: 38790203 PMC: 11120859. DOI: 10.3390/genes15050574.

The Role of HIV-1-Encoded microRNAs in Viral Replication.

Carmi O, Gotlieb Y, Shemer-Avni Y, Bentwich Z Microorganisms. 2024; 12(3).

PMID: 38543476 PMC: 10971904. DOI: 10.3390/microorganisms12030425.

MicroRNAs and long non-coding RNAs during transcriptional regulation and latency of HIV and HTLV.

Alpuche-Lazcano S, Scarborough R, Gatignol A Retrovirology. 2024; 21(1):5.

PMID: 38424561 PMC: 10905857. DOI: 10.1186/s12977-024-00637-y.

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