Extracellular ATP and UTP Exert Similar Effects on Rat Isolated Hepatocytes

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1992 Feb 1

PMID 1559136

Citations 17

Authors

S Keppens

A Vandekerckhove

H DE WULF

Affiliations

Soon will be listed here.

Abstract

1. Extracellular UTP and ATP show obvious similarities in their control of several metabolic functions of rat isolated hepatocytes. 2. They have a similar time-course and concentration-dependency for the activation of glycogen phosphorylase, the generation of inositol trisphosphate (IP3), the inhibition of glycogen synthase and the lowering of adenosine 3':5'-cyclic monophosphate (cyclic AMP) levels. 3. There is a similar synergism of the nucleotides with glucagon in activating phosphorylase. 4. They undergo a similar inhibition by phorbol myristic acid of their glycogenolytic effect. 5. The ATP and UTP effect on IP3 levels are not additive. 6. It is tentatively concluded that UTP and ATP use a common receptor.

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References

Vandenheede J, Keppens S, DE WULF H . The activation of liver phosphorylase b kinase by glucagon. FEBS Lett. 1976; 61(2):213-7. DOI: 10.1016/0014-5793(76)81040-x. View

GILMAN A . A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970; 67(1):305-12. PMC: 283204. DOI: 10.1073/pnas.67.1.305. View

Berridge M, Irvine R . Inositol phosphates and cell signalling. Nature. 1989; 341(6239):197-205. DOI: 10.1038/341197a0. View

Haussinger D, Busshardt E, Stehle T, Stoll B, Wettstein M, Gerok W . Stimulation of thromboxane release by extracellular UTP and ATP from perfused rat liver. Role of icosanoids in mediating the nucleotide responses. Eur J Biochem. 1988; 178(1):249-56. DOI: 10.1111/j.1432-1033.1988.tb14450.x. View

Burnstock G, Kennedy C . Is there a basis for distinguishing two types of P2-purinoceptor?. Gen Pharmacol. 1985; 16(5):433-40. DOI: 10.1016/0306-3623(85)90001-1. View

Gordon J . Extracellular ATP: effects, sources and fate. Biochem J. 1986; 233(2):309-19. PMC: 1153029. DOI: 10.1042/bj2330309. View

Dunn P, BLAKELEY A . Suramin: a reversible P2-purinoceptor antagonist in the mouse vas deferens. Br J Pharmacol. 1988; 93(2):243-5. PMC: 1853806. DOI: 10.1111/j.1476-5381.1988.tb11427.x. View

Charest R, Blackmore P, EXTON J . Characterization of responses of isolated rat hepatocytes to ATP and ADP. J Biol Chem. 1985; 260(29):15789-94. View

Keppens S, DE WULF H . Characterization of the biological effects of 2-methylthio-ATP on rat hepatocytes: clear-cut differences with ATP. Br J Pharmacol. 1991; 104(2):301-4. PMC: 1908554. DOI: 10.1111/j.1476-5381.1991.tb12426.x. View

10.

OConnor S, Dainty I, Leff P . Further subclassification of ATP receptors based on agonist studies. Trends Pharmacol Sci. 1991; 12(4):137-41. DOI: 10.1016/0165-6147(91)90530-6. View

11.

Pfeilschifter J . Comparison of extracellular ATP and UTP signalling in rat renal mesangial cells. No indications for the involvement of separate purino- and pyrimidino-ceptors. Biochem J. 1990; 272(2):469-72. PMC: 1149723. DOI: 10.1042/bj2720469. View

12.

Bredt D, Mourey R, Snyder S . A simple, sensitive, and specific radioreceptor assay for inositol 1,4,5-trisphosphate in biological tissues. Biochem Biophys Res Commun. 1989; 159(3):976-82. DOI: 10.1016/0006-291x(89)92204-3. View

13.

Seifert R, Schultz G . Involvement of pyrimidinoceptors in the regulation of cell functions by uridine and by uracil nucleotides. Trends Pharmacol Sci. 1989; 10(9):365-9. DOI: 10.1016/0165-6147(89)90009-6. View

14.

Lynch C, Blackmore P, Charest R, EXTON J . The relationships between receptor binding capacity for norepinephrine, angiotensin II, and vasopressin and release of inositol trisphosphate, Ca2+ mobilization, and phosphorylase activation in rat liver. Mol Pharmacol. 1985; 28(2):93-9. View

15.

Keppens S, DE WULF H . P2-purinergic control of liver glycogenolysis. Biochem J. 1985; 231(3):797-99. PMC: 1152822. DOI: 10.1042/bj2310797. View

16.

Mvumbi L, Bollen M, Stalmans W . Calcium ions and glycogen act synergistically as inhibitors of hepatic glycogen-synthase phosphatase. Biochem J. 1985; 232(3):697-704. PMC: 1152941. DOI: 10.1042/bj2320697. View

17.

Worley P, Baraban J, Supattapone S, Wilson V, Snyder S . Characterization of inositol trisphosphate receptor binding in brain. Regulation by pH and calcium. J Biol Chem. 1987; 262(25):12132-6. View

18.

Haussinger D, Stehle T, Gerok W . Actions of extracellular UTP and ATP in perfused rat liver. A comparative study. Eur J Biochem. 1987; 167(1):65-71. DOI: 10.1111/j.1432-1033.1987.tb13304.x. View

19.

Keppens S, DE WULF H . Characterization of the liver P2-purinoceptor involved in the activation of glycogen phosphorylase. Biochem J. 1986; 240(2):367-71. PMC: 1147427. DOI: 10.1042/bj2400367. View

20.

Davidson J, Wakefield I, Sohnius U, van der Merwe P, Millar R . A novel extracellular nucleotide receptor coupled to phosphoinositidase-C in pituitary cells. Endocrinology. 1990; 126(1):80-7. DOI: 10.1210/endo-126-1-80. View