Interleukin (IL)-2 and IL-3 Induce Distinct but Overlapping Responses in Murine IL-3-dependent 32D Cells Transduced with Human IL-2 Receptor Beta Chain: Involvement of Tyrosine Kinase(s) Other Than P56lck

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1992 Apr 1

PMID 1557384

Citations 15

Authors

H Otani

J P Siegel

M Erdos

J R Gnarra

M B Toledano

M Sharon

H Mostowski

M B Feinberg

J H PIERCE

W J Leonard

Affiliations

Soon will be listed here.

Abstract

We have established IL-3-dependent 32D myeloid progenitor cells stably expressing the human IL-2 receptor beta chain (IL-2R beta). Whereas parental 32D cells proliferated only in response to IL-3, the transduced cells also proliferated in response to IL-2. Transduced cells expressed high- and intermediate-affinity IL-2Rs, resulting from expression of human IL-2R beta and murine IL-2R alpha chain (IL-2R alpha). IL-2 induced phenotypic changes not induced by IL-3, including the upregulated expression of endogenous murine IL-2R alpha and IL-2R beta and an increase in cell size. Therefore, the transduced IL-2R beta was not merely coupling with the IL-3 signaling pathway. IL-3 augmented several IL-2-induced responses including the up-regulation of IL-2R alpha. Both IL-2- and IL-3-induced proliferation and IL-2 induced IL-2R alpha expression were inhibited by the tyrosine kinase inhibitor herbimycin A. Thus, both IL-2- and IL-3-mediated effects required tyrosine kinase activity. The identity of the tyrosine kinase(s) mediating the IL-2 signals in these cells is not known but cannot be p56lck, a tyrosine kinase found in T cells, since 32D-IL-2R beta cells do not express p56lck.

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