Functional Coupling of the Src-family Protein Tyrosine Kinases P59fyn and P53/56lyn with the Interleukin 2 Receptor: Implications for Redundancy and Pleiotropism in Cytokine Signal Transduction

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1993 May 1

PMID 8483935

Citations 30

Authors

N Kobayashi

T Kono

M Hatakeyama

Y Minami

T Miyazaki

R M Perlmutter

T Taniguchi

Affiliations

Soon will be listed here.

Abstract

The binding of interleukin 2 (IL-2) to the IL-2 receptor (IL-2R) induces a rapid increase in tyrosine phosphorylation of cellular proteins. In a previous study, we have shown that p56lck (lck), a src-family protein tyrosine kinase (src-PTK), physically and functionally associates with the IL-2R beta chain (IL-2R beta). To further investigate a role of src-PTKs in IL-2 signaling, we analyzed a mouse pro-B-cell line, in which lck is not expressed detectably. We observed that in this cell line, IL-2 induces activation of at least two src-PTKs, p59fyn (fyn) and p53/56lyn (lyn). Interestingly, stimulation of this cell line with IL-3 also induces activation of src-PTKs. The activation of fyn or lyn seems to be selective for stimulation with IL-2 or IL-3 since stimulation with IL-6 fails to activate them. Furthermore, we provide evidence for the physical association of fyn with IL-2R beta. Taken together with previous results, our current study suggests that different src-PTKs, each of which is expressed in a cell-type-specific manner, can participate in the IL-2 signal transduction.

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